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Aurora A antibody [35C1]

Orthogonal
Anti-Aurora A antibody [35C1] used in Western Blot (WB). GTX13824
Anti-Aurora A antibody [35C1] used in Western Blot (WB). GTX13824

Cat. No. GTX13824

Reference 16

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 35C1

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IP, IHC, PLA

Reactivity

 Human, Mouse
Package
50 μg ($399)
See all Aurora A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500-1/1,000
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
IHC Assay dependent
PLA Assay dependent

Note :

WB
This antibody detects a band of approximately 46kDa in human HeLa and mouse M-ICc12 cell lysates.
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Product Note

This antibody recognizes an epitope within the non-catalytic N-terminal domain of Aurora A. Clone 35C1 does not inhibit Aurora A kinase activity (Cremet et al. 2003).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Aurora-A

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_367503

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aurora kinase A , AIK , ARK1 , AURA , BTAK , PPP1R47 , STK15 , STK6 , STK7

Background

The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Aurora A antibody [35C1] used in Western Blot (WB). GTX13824

GTX13824 WB Image

WB analysis of Aurora A kinase recombinant protein using GTX13824 Aurora A antibody

Anti-Aurora A antibody [35C1] used in Western Blot (WB). GTX13824

GTX13824 WB Image

Orthogonal

The data was published in the journal PLoS One in 2013. PMID: 24223200

REFERENCE

Application Reference

S Kim et al. BMC Cancer 2023; 23 (1) : 1263 Endosulfine alpha maintains spindle pole integrity by recruiting Aurora A during mitosis
Application : PLA
Wuputra K et al. Int J Mol Sci 2022; 23 (14) FOXM1-CD44 Signaling Is Critical for the Acquisition of Regorafenib Resistance in Human Liver Cancer Cells.
P?rez-Fidalgo JA et al. ESMO Open 2020; 5 (5) : e000718 Aurora kinases in ovarian cancer.
Lai CH et al. Cancer Lett 2020; 472 : 97-107 A selective Aurora-A 5'-UTR siRNA inhibits tumor growth and metastasis.
Application : WB
Reactivity : Human
Wang YC et al. Exp Mol Med 2018; 50 (6) : 70 The EGF/hnRNP Q1 axis is involved in tumorigenesis via the regulation of cell cycle-related genes.
Application : IHC-P
Reactivity : Human
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REVIEW

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Application Reference
S Kim et al. BMC Cancer 2023; 23 (1):1263 Endosulfine alpha maintains spindle pole integrity by recruiting Aurora A during mitosis
Application : PLA
Wuputra K et al. Int J Mol Sci 2022; 23 (14) FOXM1-CD44 Signaling Is Critical for the Acquisition of Regorafenib Resistance in Human Liver Cancer Cells.
P?rez-Fidalgo JA et al. ESMO Open 2020; 5 (5):e000718 Aurora kinases in ovarian cancer.
Lai CH et al. Cancer Lett 2020; 472:97-107 A selective Aurora-A 5'-UTR siRNA inhibits tumor growth and metastasis.
Application : WB
Reactivity : Human
Wang YC et al. Exp Mol Med 2018; 50 (6):70 The EGF/hnRNP Q1 axis is involved in tumorigenesis via the regulation of cell cycle-related genes.
Application : IHC-P
Reactivity : Human
Richards MW et al. Proc Natl Acad Sci U S A 2016; (Epub) Structural basis of N-Myc binding by Aurora-A and its destabilization by kinase inhibitors.
Application : PLA
Reactivity : Human
Aradottir M et al. J Pathol Clin Res 2015; 1 (1):33-40 Aurora A is a prognostic marker for breast cancer arising in BRCA2 mutation carriers.
Application : IHC-P
Reactivity : Human
Wen QJ et al. Nat Med 2015; 21 (12):1473-80 Targeting megakaryocytic-induced fibrosis in myeloproliferative neoplasms by AURKA inhibition.
Application : WB
Reactivity : Mouse
Goldenson B et al. Blood 2015; 125 (13):2141-50 Aurora kinase A is required for hematopoiesis but is dispensable for murine megakaryocyte endomitosis and differentiation.
Application : WB
Reactivity : Mouse
Margret Aradottir et al. The Journal of Pathology: Clinical Research 2014; Aurora A is a prognostic marker for breast cancer arising in
Application : IHC
Reactivity : Human
Chang KC et al. PLoS One 2013; 8 (11):e79863 Ran GTPase-activating protein 1 is a therapeutic target in diffuse large B-cell lymphoma.
Chen YP et al. Int J Cancer 2013; CDKN1A-mediated responsiveness of MLL-AF4-positive acute lymphoblastic leukemia to Aurora kinase-A inhibitors.
Application : WB
Reactivity : Human
Yang G et al. Int J Cancer 2013; 133 (2):275-85 RAS promotes tumorigenesis through genomic instability induced by imbalanced expression of Aurora-A and BRCA2 in midbody during cytokinesis.
Application : ICC/IF
Reactivity : Human
Pakala SB et al. J Biol Chem 2012; 287 (48):40560-9 Signaling-dependent phosphorylation of mitotic centromere-associated kinesin regulates microtubule depolymerization and its centrosomal localization.
Yang F et al. Mod Pathol 2011; 24 (6):836-45 AURKA and BRCA2 expression highly correlate with prognosis of endometrioid ovarian carcinoma.
Yang G et al. Clin Cancer Res 2010; 16 (12):3171-81 Aurora kinase A promotes ovarian tumorigenesis through dysregulation of the cell cycle and suppression of BRCA2.
Application : ICC/IF
Reactivity : Human
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399