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Nestin antibody [10C2]

Anti-Nestin antibody [10C2] used in Flow cytometry (FACS). GTX30671
Anti-Nestin antibody [10C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30671
Anti-Nestin antibody [10C2] used in IHC (Paraffin sections) (IHC-P). GTX30671
Anti-Nestin antibody [10C2] used in Western Blot (WB). GTX30671
Anti-Nestin antibody [10C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30671

Cat. No. GTX30671

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10C2

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, MS

Reactivity

 Human, Mouse, Hamster
Package
100 μl ($449)
See all Nestin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:5000
ICC/IF 1:50 - 1:200
IHC-P 1:100 - 1:200
IHC-Fr 1:100 - 1:200
FACS 1 μg / 10⁶ cells
MS Assay dependent
Not tested in other applications.

Calculated MW

177 kDa. ( Note )

Product Note

We do not recommend use of this product for Rat,Rabbit samples.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A fragment of the human Nestin protein corresponding to residues 1464-1614. [UniProt P48681]

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_625325

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nestin , Nbla00170

Background

Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Nestin antibody [10C2] used in Flow cytometry (FACS). GTX30671

GTX30671 FACS Image

FACS (Intracellular staining) analysis of 1 x 10⁶ CHO (A) and HEK293 (B) cells using GTX30671 Nestin antibody [10C2].
Blue : Primary antibody
Orange : isotype control
Dilution : 1 μg/10⁶ cells

Anti-Nestin antibody [10C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30671

GTX30671 ICC/IF Image

ICC/IF analysis of F9 cells using GTX30671 Nestin antibody [10C2].
Green : primary antibody(left) and isotype control(right)
Blue : DAPI
Dilution : 1:50
Permibilization : 0.1% Triton X-100

Anti-Nestin antibody [10C2] used in IHC (Paraffin sections) (IHC-P). GTX30671

GTX30671 IHC-P Image

IHC-P analysis of human kidney tissue using GTX30671 Nestin antibody [10C2].
Dilution : 1:500

Anti-Nestin antibody [10C2] used in Western Blot (WB). GTX30671

GTX30671 WB Image

WB analysis of (1) rat brain tissue, and (2) human CNS progenitor cell lysate using GTX30671 Nestin antibody [10C2]. The antibody did not react with Rat, whereas, in lysate from human samples, it generated a doublet band representing the phosphorylated and the non-phosphorylated forms of Nestin protein.
Loading : 5μg
Dilution : 1:1000

Anti-Nestin antibody [10C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30671

GTX30671 ICC/IF Image

The data was published in the journal PLoS One in 2015.PMID: 26720151

REFERENCE

Application Reference

?vila-Gonz?lez D et al. Biol Sex Differ 2023; 14 (1) : 77 Increased proliferation and neuronal fate in prairie vole brain progenitor cells cultured in vitro: effects by social exposure and sexual dimorphism.
Yang JL et al. Neural Regen Res 2023; 18 (7) : 1521-1526 Transient neurogenesis in ischemic cortex from Sox2(+) astrocytes.
Application : WB
Reactivity : Mouse
Ana G Pi?a-Medina et al. Life Sci 2020; 249 : 117536 Effects of progesterone on the cell number of gliomaspheres derived from human glioblastoma cell lines.
Application : ICC/IF
Reactivity : Human
Lu P et al. J Clin Invest 2017; 127 (9) : 3287-3299 Prolonged human neural stem cell maturation supports recovery in injured rodent CNS.
Application : IHC-Fr
Reactivity : Human
Garcia-Castro IL et al. PLoS One 2015; 10 (12) : e0146082 Markers of Pluripotency in Human Amniotic Epithelial Cells and Their Differentiation to Progenitor of Cortical Neurons.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 449