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GFP antibody (HRP)

Anti-GFP antibody (HRP) used in Western Blot (WB). GTX26663

Cat. No. GTX26663

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, IHC

Reactivity

 Species independent
Package
100 μg ($399)
See all GFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000 - 1:5000
ELISA 1:10000 - 1:50000
IHC 1:500 - 1:2000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate, 0.15M NaCl, 1% BSA

Preservative

0.01% Gentamicin Sulfate

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria .

Purification

Purified by antigen-affinity chromatography.
From polyclonal serum

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_371425

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.

Research Area

DATA IMAGES

Anti-GFP antibody (HRP) used in Western Blot (WB). GTX26663

GTX26663 WB Image

Western Blot of Anti-GFP Antibody Peroxidase Conjugate (GTX26663). Lane 1: 50ng of GFP. Primary antibody: none. Secondary antibody: Anti-GFP Antibody Peroxidase Conjugate secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS 30 min at 20ºC. Predicted/Observed size: 28 kDa for GFP. Other band(s): none.

REFERENCE

Application Reference

Leen EN et al. PLoS Pathog 2016; 12 (1) : e1005379 A Conserved Interaction between a C-Terminal Motif in Norovirus VPg and the HEAT-1 Domain of eIF4G Is Essential for Translation Initiation.
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Package List Price ($)
$ 399