GeneTex

  • Country / Location Selection

United States (US)

CRYM antibody [1G7]

Overexpression
Anti-CRYM antibody [1G7] used in IHC (Paraffin sections) (IHC-P). GTX84654
Anti-CRYM antibody [1G7] used in IHC (Paraffin sections) (IHC-P). GTX84654
Anti-CRYM antibody [1G7] used in IHC (Paraffin sections) (IHC-P). GTX84654
Anti-CRYM antibody [1G7] used in Western Blot (WB). GTX84654
Anti-CRYM antibody [1G7] used in Western Blot (WB). GTX84654
Anti-CRYM antibody [1G7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84654
Anti-CRYM antibody [1G7] used in Western Blot (WB). GTX84654
Anti-CRYM antibody [1G7] used in IHC (Paraffin sections) (IHC-P). GTX84654
Anti-CRYM antibody [1G7] used in IHC (Paraffin sections) (IHC-P). GTX84654
Anti-CRYM antibody [1G7] used in Flow cytometry (FACS). GTX84654
Anti-CRYM antibody [1G7] used in Western Blot (WB). GTX84654
Anti-CRYM antibody [1G7] used in IHC (Paraffin sections) (IHC-P). GTX84654
Anti-CRYM antibody [1G7] used in IHC (Frozen sections) (IHC-Fr). GTX84654

Cat. No. GTX84654

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1G7

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

 Human, Mouse, Rat, Monkey, Marmoset
Package
100 μl ($519)
See all CRYM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100
IHC-P 1:150
IHC-Fr Assay dependent
FACS 1:100
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human CRYM (NP_001879) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_10733603

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

crystallin mu , DFNA40 , THBP

Cellular Localization

Cytoplasm

Background

Binds thyroid hormone. Presumably involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors.

Database

Research Area

DATA IMAGES

Anti-CRYM antibody [1G7] used in IHC (Paraffin sections) (IHC-P). GTX84654

GTX84654 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84654 CRYM antibody [1G7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CRYM antibody [1G7] used in IHC (Paraffin sections) (IHC-P). GTX84654

GTX84654 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84654 CRYM antibody [1G7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CRYM antibody [1G7] used in IHC (Paraffin sections) (IHC-P). GTX84654

GTX84654 IHC-P Image

IHC-P analysis of human colon tissue using GTX84654 CRYM antibody [1G7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CRYM antibody [1G7] used in Western Blot (WB). GTX84654

GTX84654 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84654 CRYM antibody [1G7].
Loading : 10 ug per lane
Dilution : 1:200

Anti-CRYM antibody [1G7] used in Western Blot (WB). GTX84654

GTX84654 WB Image

Overexpression

WB analysis of HEK293T cells transfected with CRYM plasmid (Right) or empty vector (Left) for 48 hrs using GTX84654 CRYM antibody [1G7].
Loading : 5 ug per lane

Anti-CRYM antibody [1G7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84654

GTX84654 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with CRYM plasmid using GTX84654 CRYM antibody [1G7].

Anti-CRYM antibody [1G7] used in Western Blot (WB). GTX84654

GTX84654 WB Image

WB analysis of various samples using GTX84654 CRYM antibody [1G7].
Loading : 10 ug per lane
Dilution : 1:200

Anti-CRYM antibody [1G7] used in IHC (Paraffin sections) (IHC-P). GTX84654

GTX84654 IHC-P Image

IHC-P analysis of human kidney carcinoma tissue using GTX84654 CRYM antibody [1G7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CRYM antibody [1G7] used in IHC (Paraffin sections) (IHC-P). GTX84654

GTX84654 IHC-P Image

IHC-P analysis of human breast tissue using GTX84654 CRYM antibody [1G7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CRYM antibody [1G7] used in Flow cytometry (FACS). GTX84654

GTX84654 FACS Image

FACS analysis of HEK293T cells transfected with either CRYM plasmid(Red) or empty vector control plasmid(Blue) using GTX84654 CRYM antibody [1G7].

Anti-CRYM antibody [1G7] used in Western Blot (WB). GTX84654

GTX84654 WB Image

WB analysis of various cell lines using GTX84654 CRYM antibody [1G7].
Loading : 35 ug per lane

Anti-CRYM antibody [1G7] used in IHC (Paraffin sections) (IHC-P). GTX84654

GTX84654 IHC-P Image

IHC-P analysis of human liver tissue using GTX84654 CRYM antibody [1G7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CRYM antibody [1G7] used in IHC (Frozen sections) (IHC-Fr). GTX84654

GTX84654 IHC-Fr Image

The data was published in the journal Sci Rep in 2016.PMID: 26915689

REFERENCE

Application Reference

Christian J Kinney et al. Curr Res Physiol 2021; 4 : 47-59 μ-Crystallin in Mouse Skeletal Muscle Promotes a Shift from Glycolytic toward Oxidative Metabolism.
Application : IHC-Fr
Reactivity : Mouse
Kinney C.J. et al. Current Research in Physiology 2021; 4 : 47-59 μ-Crystallin in Mouse Skeletal Muscle Promotes a Shift from Glycolytic toward Oxidative Metabolism.
Application : IHC-Fr
Reactivity : Mouse
Hosoya M et al. Sci Rep 2016; 6 : 22250 Distinct Expression Patterns Of Causative Genes Responsible For Hereditary Progressive Hearing Loss In Non-Human Primate Cochlea.
Application : IHC-Fr
Reactivity : Monkey
Submit a Reference

REVIEW

There are currently no reviews for CRYM antibody [1G7] (GTX84654). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519