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C20orf11 antibody [N1C3]

Anti-C20orf11 antibody [N1C3] used in Western Blot (WB). GTX106672
Anti-C20orf11 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106672
Anti-C20orf11 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106672

Cat. No. GTX106672

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all C20orf11 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C20orf11. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949784

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GID complex subunit 8 homolog , C20orf11 , TWA1

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-C20orf11 antibody [N1C3] used in Western Blot (WB). GTX106672

GTX106672 WB Image

Sample(30 ug whole cell lysate)
A:293T
12% SDS PAGE
GTX106672 diluted at 1:1000

Anti-C20orf11 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106672

GTX106672 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using C20orf11(GTX106672) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-C20orf11 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106672

GTX106672 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using C20orf11(GTX106672) antibody at 1:1000 dilution.

REFERENCE

Application Reference

Boldt K et al. Nat Commun 2016; 7 : 11491 An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Application : IHC-Fr
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169