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Neutrophils antibody [NIMP-R14]

Cat. No. GTX54389

Reference 2

Host

 Rat

Clonality

 Monoclonal

Clone Name

 NIMP-R14

Isotype

 IgG2b

Application

 IHC-P, IHC-Fr, FACS, ELISA, Depletion

Reactivity

 Mouse
Package
50 μg ($399)
See all Neutrophils products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS 10 μg/ml antibody / 5 x 10⁵ cells
ELISA Assay dependent
Depletion Assay dependent

Note :

IHC-P
Blocking with 20% normal rabbit serum. Use a mild or no antigen retrieval method.

IHC-Fr
Tissue was fixed with acetone.

Depletion
Neutrophil depletion. Mice were treated with NIMP-R14 given intraperitoneally at a dose of 1mg, 6h before infection.
Not tested in other applications.

Product Note

The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C. It has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 0.1% BSA

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Purified BALB/c mouse neutrophils

Purification

Protein G purified

Endotoxin

< 24 EU/mg (Determined by LAL assay)

Conjugation

Unconjugated

RRID

AB_2887856

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Gr1,Ly6G,Ly6g,lymphocyte antigen 6 complex, locus G,Gr-1,Ly-6G

Database

Research Area

REFERENCE

Application Reference

Fujihara Y et al. Tissue Eng Part A 2019; (Epub) Influence of Damage-Associated Molecular Patterns from Chondrocytes in Tissue-Engineered Cartilage.
Application : IHC-P
Reactivity : Mouse
McGovern T et al. J Immunol 2016; 197 (1) : 266-77 CysLT1 Receptor Is Protective against Oxidative Stress in a Model of Irritant-Induced Asthma.
Application : IHC-P
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Package List Price ($)
$ 399