Histone H2A.XS139ph (phospho Ser139) antibody [GT2311]

Name: Histone H2A.XS139ph (phospho Ser139) antibody [GT2311]
Brand: GeneTex
SKU: GTX628789
Price: 399.00 USD

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] used in Immunoprecipitation (IP). GTX628789

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] used in Western Blot (WB). GTX628789

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] used in IHC (Paraffin sections) (IHC-P). GTX628789

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628789

Cat. No. GTX628789

Reference 18

Host	Mouse
Clonality	Monoclonal
Clone Name	GT2311
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Histone H2A.X products

View product citations for antibody GTX628789 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

HCT116 (30μM cisplatin treatment for 24hr) , NIH-3T3 (30μM cisplatin treatment for 24hr) , PC-12 (30uM cisplatin treatment for 24h) , U2OS treated UV (50 J/m2)

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser139 of human Histone H2A.X. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888051

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

,H2A histone family member X,gamma H2A.X,gamma H2AX,H2A.X,H2A/X,H2AX,H2AFX,

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]

Database

Gene ID: 3014 H2AFX
UniProt: P16104 H2AFX

Research Area

DATA IMAGES

GTX628789 IP Image

Histone H2A.X (phospho S139) antibody immunoprecipitates histone H2A.X (phospho S139) protein in IP experiments. IP Sample: 500 μg HCT116 with CPT 30 μM treatment 24 hr whole cell lysate/extract A. 30 μg HCT116 whole with CPT 30 uM treatment cell lysate/extract B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of histone H2A.X (phospho S139) protein by 2 μg histone H2A.X (phospho S139) antibody (GTX628789) 15% SDS-PAGE The immunoprecipitated histone H2A.X (phospho S139) protein was detected by Human histone H2A.X (phospho S139) antibody (GTX628789) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

GTX628789 WB Image

Histone H2A.X (phospho S139) antibody [GT2311] detects H2AFX protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract (untreated)
B. 30 μg NIH-3T3 whole cell lysate/extract (30μM cisplatin treatment for 24hr)
15% SDS-PAGE
Histone H2A.X (phospho S139) antibody [GT2311] (GTX628789) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX628789 IHC-P Image

Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] (GTX628789) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX628789 ICC/IF Image

Histone H2A.X (phospho Ser139) antibody detects H2AFX protein at nuclear by confocal immunofluorescent analysis.
Sample: 10μM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min.
Red: H2A.X protein stained by Histone H2A.X (phospho Ser139) antibody (GTX628789) diluted at 1:500.
Green: alpha Tubulin antibody (GTX102078) diluted at 1:1000.
Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]

GTX628789 WB Image

Histone H2A.X (phospho S139) antibody [GT2311] detects Histone H2A.X (phospho S139) [GT2311] protein by western blot analysis. Un-treated (-) and treated (+, 30 μM Cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X (phospho S139) antibody [GT2311] (GTX628789) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX628789 ICC/IF Image

Histone H2A.X antibody detects H2AFX protein at nuclear by immunofluorescent analysis. Sample: 10μM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: H2AFX protein stained by Histone H2A.Xantibody (GTX628789) diluted at 1:500. Blue: Hoechst 33342 staining.

GTX628789 WB Image

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein by western blot analysis. Un-treated (-) and treated (+, 50 J/m2 UV treatment) U2OS whole cell extracts (16 μg) were separated by 12%-15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX628789) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX628789 WB Image

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] (GTX628789) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX628789 WB Image

The data was published in the journal Sci Rep in 2020.PMID: 31980707

GTX628789 WB Image

The data was published in the 2019 in Int J Mol Sci. PMID: 31717306

GTX628789 ICC/IF Image

The data was published in the 2019 in Int J Mol Sci. PMID: 31717306

GTX628789 WB Image

The data was published in the journal PLoS Negl Trop Dis in 2017. PMID: 28783752

GTX628789 WB Image

The data was published in the journal Front Physiol in 2019. PMID: 31681015

REFERENCE

Application Reference

Lin-Sheng Yu et al. Cancers (Basel) 2021; 13 (16) Tissue Architecture Influences the Biological Effectiveness of Boron Neutron Capture Therapy in In Vitro/In Silico Three-Dimensional Self-Assembly Cell Models of Pancreatic Cancers.

Application : ICC/IF

Reactivity : Human

Tomita T et al. Nat Commun 2021; 12 (1) : 3655 Extracellular mRNA transported to the nucleus exerts translation-independent function.

Application : ICC/IF

Reactivity : Human

Chen TY et al. Cell Death Differ 2021; (Epub) Genotoxic stress-activated DNA-PK-p53 cascade and autophagy cooperatively induce ciliogenesis to maintain the DNA damage response.

Application : WB, ICC/IF

Reactivity : Human

Li G et al. J Cell Sci 2021; 134 (2) Matrix stiffness regulates α-TAT1-mediated acetylation of α-tubulin and promotes silica-induced epithelial-mesenchymal transition via DNA damage.

Application : WB, IHC-P

Reactivity : Human

Lin BZ et al. Molecules 2020; 25 (16) Involvement of Differentially Expressed microRNAs in the PEGylated Liposome Encapsulated ¹⁸⁸Rhenium-Mediated Suppression of Orthotopic Hypopharyngeal Tumor.

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Application Reference

Application : ICC/IF

Reactivity : Human

Tomita T et al. Nat Commun 2021; 12 (1):3655 Extracellular mRNA transported to the nucleus exerts translation-independent function.

Application : ICC/IF

Reactivity : Human

Chen TY et al. Cell Death Differ 2021; (Epub) Genotoxic stress-activated DNA-PK-p53 cascade and autophagy cooperatively induce ciliogenesis to maintain the DNA damage response.

Application : WB, ICC/IF

Reactivity : Human

Li G et al. J Cell Sci 2021; 134 (2) Matrix stiffness regulates α-TAT1-mediated acetylation of α-tubulin and promotes silica-induced epithelial-mesenchymal transition via DNA damage.

Application : WB, IHC-P