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HMGB1 antibody

KO/KD Orthogonal Overexpression
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277
Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277
Anti-HMGB1 antibody used in IHC (Whole mount) (IHC-Wm). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277
Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277
Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

Cat. No. GTX101277

Reference 22
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm, IHC, IHC (Free Floating)

Reactivity

 Human, Mouse, Rat, Zebrafish, Pig
Package
100 μl ($399),
25 μl ($169)
See all HMGB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IHC Assay dependent
IHC (Free Floating) Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

NIH-3T3 , JC , BCL-1 , C2C12 , Raw264.7 , PC-12 , HMGB1-transfected 293T

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950499

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

high mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1

Cellular Localization

Nucleus

Background

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Database

Research Area

DATA IMAGES

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX101277) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HMGB1 antibody (GTX101277) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
HMGB1 antibody (GTX101277) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus in mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
HMGB1 antibody (GTX101277) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX101277) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

HMGB1 antibody detects HMGB1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody (GTX101277) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX101277) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277

GTX101277 ICC/IF Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX101277) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
HMGB1 antibody (GTX101277) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277

GTX101277 ICC/IF Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX101277) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
HMGB1 stained by HMGB1 antibody (GTX101277) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
HMGB1 stained by HMGB1 antibody (GTX101277) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
HMGB1 stained by HMGB1 antibody (GTX101277) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX101277) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

HMGB1 antibody detects HMGB1 protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
B. 30 μg JC whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysate/extract
D. 30 μg C2C12 whole cell lysate/extract
E. 30 μg Raw264.7 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody (GTX101277) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody used in IHC (Whole mount) (IHC-Wm). GTX101277

GTX101277 IHC-Wm Image

HMGB1 antibody detects Hmgb1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Hmgb1 stained by HMGB1 antibody (GTX101277) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using HMGB1 antibody (GTX101277) at 1:300 dilution.

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

Various tissue extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX101277) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (GI: gastrointestinal)

Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277

GTX101277 ICC/IF Image

HMGB1 antibody detects HMGB1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX101277) diluted at 1:1000.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Scale bar = 10 μm.

Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277

GTX101277 ICC/IF Image

Orthogonal

The data was published in the journal Oncotarget in 2018. PMID: 30279967

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

Orthogonal

The data was published in the 2016 in Nat Commun. PMID: 27329339

REFERENCE

Application Reference

Wen J et al. Int Immunopharmacol 2023; 119 : 110259 Knockdown of HMGB1 inhibits the crosstalk between oral squamous cell carcinoma cells and tumor-associated macrophages
Application : WB
Reactivity : Human
Zhengke Li et al. FASEB J 2021; 35 (5) : e21373 ATR prevents Ca2+ overload-induced necrotic cell death through phosphorylation-mediated inactivation of PARP1 without DNA damage signaling.
Application : WB
Reactivity : Human
Lin C.Y. et al. Natural Product Communications 2021; (Epub) Tanshinone IIA Shows Higher Antiproliferative Activities than Sinapic Acid in 4 Cancer Cell Lines and Simultaneously Induces Apoptosis and Necroptosis in Human Lung Cancer A549 Cells.
Application : WB
Reactivity : Human
Sixto-L?pez Y et al. Int J Mol Sci 2020; 21 (16) N-(2'-Hydroxyphenyl)-2-Propylpentanamide (HO-AAVPA) Inhibits HDAC1 and Increases the Translocation of HMGB1 Levels in Human Cervical Cancer Cells.
Kanno Y et al. Arthritis Res Ther 2020; 22 (1) : 76 Alternatively Activated Macrophages Are Associated With the α2AP Production That Occurs With the Development of Dermal Fibrosis : The Role of Alternatively Activated Macrophages on the Development of Fibrosis.
Application : WB
Reactivity : Mouse
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Submit a Reference

REVIEW

HMGB1 antibody Cat. No. GTX101277

Rating
( Average 5 based on 1 users reviews)
Application
IHC (Free Floating)(IHC (Free Floating))
( Average 5 based on 1 users reviews)
Anti-HMGB1 antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX101277
Date : Anonymous submitted on 14-Feb-2023
Score :
Application Tested : IHC (Free Floating)
Sample Species : Ms
Sample : adult mouse cerebral cortex tissue section
Blocking : 1% BSA in PBS, 25°C, 0.5Hr
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : 0.3% Triton X-100 / No antigen retireval
Primary Antibody : 1:500, 16°C, 4Hr
Submit a Review
Application Reference
Wen J et al. Int Immunopharmacol 2023; 119:110259 Knockdown of HMGB1 inhibits the crosstalk between oral squamous cell carcinoma cells and tumor-associated macrophages
Application : WB
Reactivity : Human
Zhengke Li et al. FASEB J 2021; 35 (5):e21373 ATR prevents Ca2+ overload-induced necrotic cell death through phosphorylation-mediated inactivation of PARP1 without DNA damage signaling.
Application : WB
Reactivity : Human
Lin C.Y. et al. Natural Product Communications 2021; (Epub) Tanshinone IIA Shows Higher Antiproliferative Activities than Sinapic Acid in 4 Cancer Cell Lines and Simultaneously Induces Apoptosis and Necroptosis in Human Lung Cancer A549 Cells.
Application : WB
Reactivity : Human
Sixto-L?pez Y et al. Int J Mol Sci 2020; 21 (16) N-(2'-Hydroxyphenyl)-2-Propylpentanamide (HO-AAVPA) Inhibits HDAC1 and Increases the Translocation of HMGB1 Levels in Human Cervical Cancer Cells.
Kanno Y et al. Arthritis Res Ther 2020; 22 (1):76 Alternatively Activated Macrophages Are Associated With the α2AP Production That Occurs With the Development of Dermal Fibrosis : The Role of Alternatively Activated Macrophages on the Development of Fibrosis.
Application : WB
Reactivity : Mouse
Huang L et al. Aging (Albany NY) 2019; 11 (17):6674-6690 RPN2 promotes metastasis of hepatocellular carcinoma cell and inhibits autophagy via STAT3 and NF-κB pathways.
D繹rsam B et al. Proc Natl Acad Sci U S A 2018; 115 (17):E4061-E4070 PARP-1 protects against colorectal tumor induction, but promotes inflammation-driven colorectal tumor progression.
Contis-Montes de Oca A et al. Oncotarget 2018; 9 (70):33368-33381 N-(2'-Hydroxyphenyl)-2-propylpentanamide (OH-VPA), a Histone Deacetylase Inhibitor, Induces the Release of Nuclear HMGB1 and Modifies ROS Levels in HeLa Cells.
Coleman LG Jr et al. Brain Behav Immun 2017; (Epub) HMGB1/IL-1β complexes regulate neuroimmune responses in alcoholism.
Application : WB
Reactivity : Human
Masola V et al. FASEB J 2017; (Epub) Heparanase regulates the * polarization of renal macrophages and their crosstalk with renal epithelial tubular cells after ischemia/reperfusion injury.
Application : ICC/IF
Reactivity : Human
Liu PL et al. PLoS One 2017; 12 (7):e0180844 MicroRNA-200c inhibits epithelial-mesenchymal transition, invasion, and migration of lung cancer by targeting HMGB1.
Application : WB, ICC/IF, IHC-P
Reactivity : Human
Lin WC et al. RSC Adv. 2017; 7:21234-21251 Anti-inflammatory activity of Sanghuangporus sanghuang by suppressing the TLR4-mediated PI3K/AKT/mTOR/IKKβ signaling pathway.
Application : WB
Reactivity : Mouse
Lin CY et al. Cell Death Discov 2016; 2:16065 Simultaneous induction of apoptosis and necroptosis by Tanshinone IIA in human hepatocellular carcinoma HepG2 cells.
Application : WB
Reactivity : Human
Dick MS et al. Nat Commun 2016; 7:11929 ASC filament formation serves as a signal amplification mechanism for inflammasomes.
Application : WB
Reactivity : Mouse
Bhatia A et al. Mol Ther Methods Clin Dev 2016; 3:16041 Dual therapeutic functions of F-5 fragment in burn wounds: preventing wound progression and promoting wound healing in pigs.
Application : IHC-P
Reactivity : Pig
Sundararaman B et al. Mol Cell 2016; 61 (6):903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : ICC/IF
Reactivity : Human
Lin TJ et al. Mol Cancer 2015; 14:174 Shikonin-enhanced cell immunogenicity of tumor vaccine is mediated by the differential effects of DAMP components.
Application : WB
Reactivity : Mouse
Juranek JK et al. Front Cell Neurosci 2015; 9:485 Receptor for Advanced Glycation End Products and its Inflammatory Ligands are Upregulated in Amyotrophic Lateral Sclerosis.
Application : WB
Reactivity : Human
Ruhl S et al. Eur J Immunol 2015; 45 (10):2927-36 Caspase-11 activates a canonical NLRP3 inflammasome by promoting K(+) efflux.
Application : WB
Reactivity : Human
Xu YF et al. World J Gastroenterol 2015; 21 (11):3256-65 High-mobility group box 1 expression and lymph node metastasis in intrahepatic cholangiocarcinoma.
Application : IHC
Reactivity : Human
Lee SM et al. Int J Cardiol 2015; 190:353-9 Amitriptyline pharmacologically preconditions rat hearts against cardiac ischemic-reperfusion injury.
Application : WB
Reactivity : Rat
Kayagaki N et al. Nature 2011; 479 (7371):117-21 Non-canonical inflammasome activation targets caspase-11.
Application : WB
Reactivity : Mouse
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