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IKAP antibody [C1C2], Internal

Anti-IKAP antibody [C1C2], Internal used in Western Blot (WB). GTX110274
Anti-IKAP antibody [C1C2], Internal used in Western Blot (WB). GTX110274
Anti-IKAP antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110274

Cat. No. GTX110274

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all IKAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

150 kDa. ( Note )

Positive Control

HeLa , HepG2 , GL261 , Raw264.7

Predict Reactivity

Rat, Rabbit(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IKAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10724877

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

elongator complex protein 1 , DYS , FD , IKAP , IKBKAP , IKI3 , TOT1

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a scaffold protein and a regulator for 3 different kinases involved in proinflammatory signaling. This encoded protein can bind NF-kappa-B-inducing kinase (NIK) and IKKs through separate domains and assemble them into an active kinase complex. Mutations in this gene have been associated with familial dysautonomia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IKAP antibody [C1C2], Internal used in Western Blot (WB). GTX110274

GTX110274 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
B: HepG2
5% SDS PAGE
GTX110274 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IKAP antibody [C1C2], Internal used in Western Blot (WB). GTX110274

GTX110274 WB Image

IKAP antibody [C1C2], Internal detects IKAP protein by western blot analysis.
A. 30 μg GL261 whole cell lysate/extract
B. 30 μg Raw264.7 whole cell lysate/extract
5% SDS-PAGE
IKAP antibody [C1C2], Internal (GTX110274) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IKAP antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110274

GTX110274 ICC/IF Image

IKAP antibody [C1C2], Internal detects IKAP protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: IKAP protein stained by IKBKAP antibody [C1C2], Internal (GTX110274) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE

Application Reference

Zeltner N et al. Nat Med 2016; 22 (12) : 1421-1427 Capturing the biology of disease severity in a PSC-based model of familial dysautonomia.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169