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p70 S6K (phospho Thr229) antibody

Orthogonal
Anti-p70 S6K (phospho Thr229) antibody used in Western Blot (WB). GTX25231
Anti-p70 S6K (phospho Thr229) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25231
Anti-p70 S6K (phospho Thr229) antibody used in Western Blot (WB). GTX25231
Anti-p70 S6K (phospho Thr229) antibody used in Western Blot (WB). GTX25231

Cat. No. GTX25231

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Rat
Package
50 μl ($399)
See all p70 S6K products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:250
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human p70-S6K that contains threonine 229. The sequence is conserved in rat.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380486

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein S6 kinase B1 , PS6K , S6K , S6K-beta-1 , S6K1 , STK14A , p70 S6KA , p70(S6K)-alpha , p70-S6K , p70-alpha

Cellular Localization

Mitochondrion outer membrane,Mitochondrion,Cytoplasm

Background

This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]

Database

Research Area

DATA IMAGES

Anti-p70 S6K (phospho Thr229) antibody used in Western Blot (WB). GTX25231

GTX25231 WB Image

Orthogonal

WB (peptide competition) analysis of Jurkat cells using GTX25231 p70 S6K (phospho Thr229) antibody prior incubated with the non-phosphorylated peptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphothreonine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.

Anti-p70 S6K (phospho Thr229) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25231

GTX25231 ICC/IF Image

Orthogonal

ICC/IF analysis of A431 cells treated with 200 ng/ml of EGF for 10 minutes using GTX25231 p70 S6K (phospho Thr229) antibody. Panel e is untreated cell with nuclear localization. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes

Anti-p70 S6K (phospho Thr229) antibody used in Western Blot (WB). GTX25231

GTX25231 WB Image

Orthogonal

The data was published in the journal Life Sci Alliance in 2019.PMID: 30923191

Anti-p70 S6K (phospho Thr229) antibody used in Western Blot (WB). GTX25231

GTX25231 WB Image

Orthogonal

The data was published in the journal Life Sci Alliance in 2019.PMID: 30923191

REFERENCE

Application Reference

Heberle AM et al. Life Sci Alliance 2019; 2 (2) The PI3K and MAPK/p38 pathways control stress granule assembly in a hierarchical manner.
Yang GS et al. Mol Med Rep 2018; 17 (4) : 5821-5829 mTOR is involved in stroke-induced seizures and the anti-seizure effect of mild hypothermia.
Application : WB
Reactivity : Rat
Dalle Pezze P et al. Nat Commun 2016; 7 : 13254 A systems study reveals concurrent activation of AMPK and mTOR by amino acids.
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399