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Iba1 (isoform 3) antibody, Internal

Anti-Iba1 (isoform 3) antibody, Internal used in Western Blot (WB). GTX89367
Anti-Iba1 (isoform 3) antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89367

Cat. No. GTX89367

Reference 2

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all Iba1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-1.5μg/ml
IHC-P 2-4μg/ml
IHC-Fr Assay dependent

Note :

IHC-P
Mouse Brain shows staining of astrocytes.
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89367-PEP).

Product Note

This antibody is expected to recognise isoform 3 (NP_001614.3) only.

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-NKQFLDDPKYSSDED, from the internal region of the protein sequence according to NP_001614.3.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10722677

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

allograft inflammatory factor 1 , AIF-1 , IBA1 , IRT-1 , IRT1

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-Iba1 (isoform 3) antibody, Internal used in Western Blot (WB). GTX89367

GTX89367 WB Image

WB analysis of human frontal cortex lysate using GTX89367 Iba1 (isoform 3) antibody, Internal.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

Anti-Iba1 (isoform 3) antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89367

GTX89367 IHC-P Image

IHC-P analysis of mouse brain using GTX89367 Iba1 (isoform 3) antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml

REFERENCE

Application Reference

Schiaffino L et al. Sci Rep 2018; 8 (1) : 12875 Acetylation state of RelA modulated by epigenetic drugs prolongs survival and induces a neuroprotective effect on ALS murine model.
Lee JY et al. Sci Rep 2017; 7 : 45646 Important roles of Vilse in dendritic architecture and synaptic plasticity.
Application : IHC-Fr
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399