GeneTex

  • Country / Location Selection

United States (US)

Insulin antibody

Orthogonal
Anti-Insulin antibody used in IHC (Paraffin sections) (IHC-P). GTX27842

Cat. No. GTX27842

Reference 14

Host

 Guinea pig

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
500 μl ($399)
See all Insulin products

APPLICATION

Application Note

For IHC/ICC: Use at a dilution of 1:50-1:100. Prolonged fixation in buffered formalin can destroy the epitope. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

12 kDa. ( Note )

Product Note

This antibody stains beta (b) cells of the Langerhan's islets in human pancreas.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.2% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human protein

Purification

Neat Serum

Conjugation

Unconjugated

RRID

AB_371884

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin , IDDM , IDDM1 , IDDM2 , ILPR , IRDN , MODY10

Cellular Localization

Secreted

Background

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-Insulin antibody used in IHC (Paraffin sections) (IHC-P). GTX27842

GTX27842 IHC-P Image

Insulin antibody detects Insulin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreas.
Insulin stained by Insulin antibody (GTX27842) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Daian LM et al. Int J Mol Sci 2023; 24 (3) Modulation of Unfolded Protein Response Restores Survival and Function of β-Cells Exposed to the Endocrine Disruptor Bisphenol A.
Application : ICC/IF
Reactivity : Mouse
Katz LS et al. Mol Metab 2023; 79 : 101848 Transcriptional activation of the Myc gene by glucose in β-cells requires a ChREBP-dependent 3-D chromatin interaction between the Myc and Pvt1 genes.
Morales-Reyes I et al. Islets 2022; 14 (1) : 149-163 Impact of biotin supplemented diet on mouse pancreatic islet β-cell mass expansion and glucose induced electrical activity.
Application : IHC-P
Reactivity : Mouse
Lee SJ et al. Cell Transplant 2020; 29 : 963689720913876 Donor-Specific Regulatory T Cell-Mediated Immune Tolerance in an Intrahepatic Murine Allogeneic Islet Transplantation Model with Short-Term Anti-CD154 mAb Single Treatment.
Application : IHC-P
Reactivity : Mouse
Nteeba J et al. J Endocrinol 2019; (Epub) Pancreatic prolactin receptor signaling regulates maternal glucose homeostasis.
Show More +
Submit a Reference

REVIEW

There are currently no reviews for Insulin antibody (GTX27842). Be the first to share your experience with this product.
Submit a Review
Application Reference
Daian LM et al. Int J Mol Sci 2023; 24 (3) Modulation of Unfolded Protein Response Restores Survival and Function of β-Cells Exposed to the Endocrine Disruptor Bisphenol A.
Application : ICC/IF
Reactivity : Mouse
Katz LS et al. Mol Metab 2023; 79:101848 Transcriptional activation of the Myc gene by glucose in β-cells requires a ChREBP-dependent 3-D chromatin interaction between the Myc and Pvt1 genes.
Morales-Reyes I et al. Islets 2022; 14 (1):149-163 Impact of biotin supplemented diet on mouse pancreatic islet β-cell mass expansion and glucose induced electrical activity.
Application : IHC-P
Reactivity : Mouse
Lee SJ et al. Cell Transplant 2020; 29:963689720913876 Donor-Specific Regulatory T Cell-Mediated Immune Tolerance in an Intrahepatic Murine Allogeneic Islet Transplantation Model with Short-Term Anti-CD154 mAb Single Treatment.
Application : IHC-P
Reactivity : Mouse
Nteeba J et al. J Endocrinol 2019; (Epub) Pancreatic prolactin receptor signaling regulates maternal glucose homeostasis.
Mouna El Mehdi et al. bioRxiv 2019; (Epub) Glucose homeostasis is impaired in mice deficient for the neuropeptide 26RFa (QRFP).
Application : IHC-P
Reactivity : Mouse
Chien HJ et al. Am J Physiol Gastrointest Liver Physiol 2019; Human pancreatic afferent and efferent nerves: mapping and 3-D illustration of exocrine, endocrine, and adipose innervation.
Reactivity : Mouse
Tang SC et al. Diabetologia 2017; (Epub) Pancreatic neuro-insular network in young mice revealed by 3D panoramic histology.
Tang SC et al. Diabetologia 2017; (Epub) Human pancreatic neuro-insular network in health and fatty infiltration.
Yamashita-Sugahara Y et al. Sci Rep 2016; 6:35908 An inhibitor of fibroblast growth factor receptor-1 (FGFR1) promotes late-stage terminal differentiation from NGN3+ pancreatic endocrine progenitors.
Application : ICC/IF
Reactivity : Human
Lin PY et al. Am J Physiol Gastrointest Liver Physiol 2016;:ajpgi.00071.2016 PanIN-associated pericyte, glial, and islet remodeling in mice revealed by 3-D pancreatic duct lesion histology.
Masjkur J et al. J Biol Chem 2014; hes3 is expressed in the adult pancreatic islet and regulates gene expression, cell growth, and * release.
Application : ICC/IF
Reactivity : Mouse
Nagaishi K et al. Hepatology 2013; Mesenchymal stem cell therapy ameliorates diabetic hepatocyte damage in mice by inhibiting infiltration of bone marrow-derived cells.
Application : ICC/IF, IHC-Fr
Reactivity : Human, Mouse
Liu Y et al. PLoS One 2010; 5 (10):e13533 *-independent recombination in the rip-creer mouse.
Application : IHC-Fr
Reactivity : Mouse
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399