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IRS1 (phospho Tyr896) antibody

Orthogonal
Anti-IRS1 (phospho Tyr896) antibody used in Western Blot (WB). GTX24873
Anti-IRS1 (phospho Tyr896) antibody used in IHC (Paraffin sections) (IHC-P). GTX24873

Cat. No. GTX24873

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
50 μl ($399)
See all IRS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:10-1:100
Not tested in other applications.

Calculated MW

132 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of IRS-1 that contains tyrosine 896. The sequence is conserved among multiple species including human, mouse and rat.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380454

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin receptor substrate 1 , HIRS-1

Background

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-IRS1 (phospho Tyr896) antibody used in Western Blot (WB). GTX24873

GTX24873 WB Image

Orthogonal

WB analysis of samples using GTX24873 IRS1 (phospho Tyr896) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

Anti-IRS1 (phospho Tyr896) antibody used in IHC (Paraffin sections) (IHC-P). GTX24873

GTX24873 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX24873 IRS1 (phospho Tyr896) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

REFERENCE

Application Reference

Renna LV et al. PLoS One 2017; 12 (9) : e0184987 receptor and post-receptor abnormalities contribute to * resistance in myotonic dystrophy type 1 and type 2 skeletal muscle.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399