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GPR32 antibody

KO/KD
Anti-GPR32 antibody used in IHC (Paraffin sections) (IHC-P). GTX71225
Anti-GPR32 antibody used in IHC (Paraffin sections) (IHC-P). GTX71225
Anti-GPR32 antibody used in IHC (Paraffin sections) (IHC-P). GTX71225
Anti-GPR32 antibody used in IHC (Paraffin sections) (IHC-P). GTX71225
Anti-GPR32 antibody used in IHC (Paraffin sections) (IHC-P). GTX71225
Anti-GPR32 antibody used in IHC (Paraffin sections) (IHC-P). GTX71225

Cat. No. GTX71225

Reference 11

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS, Neutralizing/Inhibition

Reactivity

 Human, Rat
Package
25 μg ($299)
See all GPR32 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 38 μg/ml
FACS Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Product Note

Knockdown/Knockout validation was supported by references (PMID:35856414)

Predict Reactivity

Marmoset(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic 16 amino acid peptide from 3rd extracellular domain of human GPR32.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_371030

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

, G proteincoupled receptor 32 , GPR32 , RVDR1

Cellular Localization

Cell membrane

Background

This gene is intronless and encodes a member of the G-protein coupled receptor 1 family. The encoded protein binds to resolvin D1 and lipoxin A4 and has been linked to pulmonary inflammation. A related pseudogene has been identified on chromosome 19. [provided by RefSeq, Nov 2012]

Database

Research Area

DATA IMAGES

Anti-GPR32 antibody used in IHC (Paraffin sections) (IHC-P). GTX71225

GTX71225 IHC-P Image

IHC-P analysis of lung, non small cell carcinoma tissue using GTX71225 GPR32 antibody.

Anti-GPR32 antibody used in IHC (Paraffin sections) (IHC-P). GTX71225

GTX71225 IHC-P Image

IHC-P analysis of human brain, neurons and glia tissue using GTX71225 GPR32 antibody.
Antigen retrieval : Heat-induced antigen retrieval

Anti-GPR32 antibody used in IHC (Paraffin sections) (IHC-P). GTX71225

GTX71225 IHC-P Image

IHC-P analysis of brain, medulla, spinal trigeminal nucleus tissue using GTX71225 GPR32 antibody.

Anti-GPR32 antibody used in IHC (Paraffin sections) (IHC-P). GTX71225

GTX71225 IHC-P Image

IHC-P analysis of brain, glioblastoma tissue using GTX71225 GPR32 antibody.

Anti-GPR32 antibody used in IHC (Paraffin sections) (IHC-P). GTX71225

GTX71225 IHC-P Image

IHC-P analysis of human ovary, carcinoma tissue using GTX71225 GPR32 antibody.
Antigen retrieval : Heat-induced antigen retrieval

Anti-GPR32 antibody used in IHC (Paraffin sections) (IHC-P). GTX71225

GTX71225 IHC-P Image

IHC-P analysis of human brain, glioblastoma tissue using GTX71225 GPR32 antibody.
Antigen retrieval : Heat-induced antigen retrieval

REFERENCE

Application Reference

Wang L et al. Mol Med Rep 2022; 26 (3) Protective effect of 17S?epoxy?docosapentaenoic acid against dextran sulfate sodium induced ulcerative colitis in BALB/c mice.
Application : FACS
Reactivity : Human
Chiurchiu V et al. FASEB J 2018; : fj201801017R Resolution of inflammation is altered in chronic heart failure and entails a dysfunctional responsiveness of T lymphocytes.
Application : WB
Reactivity : Human
Chattopadhyay R et al. Free Radic Biol Med 2018; 117 : 119-131 Resolvin D1 blocks H2O2-mediated inhibitory crosstalk between SHP2 and PP2A and suppresses endothelial-monocyte interactions.
Application : Neutralizing/Inhibition
Chattopadhyay R et al. Redox Biol 2017; 12 : 438-455 Resolvin D1 via prevention of ROS-mediated SHP2 inactivation protects endothelial adherens junction integrity and barrier function.
Sulciner ML et al. J Exp Med 2017; (Epub) Resolvins suppress tumor growth and enhance cancer therapy.
Application : IHC-P
Reactivity : Human
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REVIEW

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Application Reference
Wang L et al. Mol Med Rep 2022; 26 (3) Protective effect of 17S?epoxy?docosapentaenoic acid against dextran sulfate sodium induced ulcerative colitis in BALB/c mice.
Application : FACS
Reactivity : Human
Chiurchiu V et al. FASEB J 2018;:fj201801017R Resolution of inflammation is altered in chronic heart failure and entails a dysfunctional responsiveness of T lymphocytes.
Application : WB
Reactivity : Human
Chattopadhyay R et al. Free Radic Biol Med 2018; 117:119-131 Resolvin D1 blocks H2O2-mediated inhibitory crosstalk between SHP2 and PP2A and suppresses endothelial-monocyte interactions.
Application : Neutralizing/Inhibition
Chattopadhyay R et al. Redox Biol 2017; 12:438-455 Resolvin D1 via prevention of ROS-mediated SHP2 inactivation protects endothelial adherens junction integrity and barrier function.
Sulciner ML et al. J Exp Med 2017; (Epub) Resolvins suppress tumor growth and enhance cancer therapy.
Application : IHC-P
Reactivity : Human
Chiurchiu V et al. Sci Transl Med 2016; 8 (353):353ra111 Proresolving lipid mediators resolvin D1, resolvin D2, and maresin 1 are critical in modulating T cell responses.
Application : WB, Neutralizing/Inhibition
Reactivity : Human
Croasdell A et al. Am J Physiol Lung Cell Mol Physiol 2015; 309 (8):L888-901 Resolvins attenuate inflammation and promote resolution in cigarette smoke-exposed human macrophages.
Hsiao HM et al. J Immunol 2014; 193 (10):1125131 Resolvin D1 Attenuates Polyinosinic-Polycytidylic Acid-Induced Inflammatory Signaling in Human Airway Epithelial Cells via TAK1.
Application : FACS
Reactivity : Human
Norling LV et al. Arterioscler Thromb Vasc Biol 2012; 32 (8):25781 Resolvin D1 limits polymorphonuclear leukocyte recruitment to inflammatory loci: receptor-dependent actions.
Dartt DA et al. J Immunol 2011; 186 (7):4455-66 Conjunctival goblet cell secretion stimulated by leukotrienes is reduced by resolvins D1 and E1 to promote resolution of inflammation.
Application : IHC-P
Reactivity : Rat
Krishnamoorthy S et al. Proc Natl Acad Sci U S A 2010; 107 (4):1660-5 Resolvin D1 binds human phagocytes with evidence for proresolving receptors.
Application : FACS
Reactivity : Human
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 299