GABARAPL2 antibody

Name: GABARAPL2 antibody
Brand: GeneTex
SKU: GTX102006
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-GABARAPL2 antibody used in Western Blot (WB). GTX102006

Anti-GABARAPL2 antibody used in Immunohistochemistry (IHC). GTX102006

Anti-GABARAPL2 antibody used in IHC (Whole mount) (IHC-Wm). GTX102006

Anti-GABARAPL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX102006

Cat. No. GTX102006

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, IHC-Wm, IHC
Reactivity	Human, Mouse, Rat, Zebrafish
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX102006 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000
IHC-Wm	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929

Predict Reactivity

Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GABARAPL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240874

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GABA type A receptor associated protein like 2 , ATG8 , ATG8C , GATE-16 , GATE16 , GEF-2 , GEF2

Background

Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1.

Database

Gene ID: 11345 GABARAPL2
UniProt: P60520 GABARAPL2

Research Area

DATA IMAGES

GTX102006 WB Image

GABARAPL2 antibody detects GABARAPL2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAPL2 antibody (GTX102006) diluted at a dilution of 1:1500.

GTX102006 IHC Image

GABARAPL2 antibody detects Gabarapl2 protein on photoreceptors by immunohistochemical analysis Sample: Agarose-embedded zebrafish embryo GABARAPL2 antibody (GTX102006) dilution: 1:200. Image provided with permission courtesy of Dr. T. Schilling at UC, Irvine.

GTX102006 IHC-Wm Image

GABARAPL2 antibody detects Gabarapl2 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
GABARAPL2 antibody (GTX102006) dilution: 1:50.

GTX102006 IHC-P Image

GABARAPL2 antibody detects GABARAPL2 protein at cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
GABARAPL2 antibody (GTX102006) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102006 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using GABARAPL2(GTX102006) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Markmiller S et al. Cell 2018; 172 (3) : 590-604.e13 Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules.

Reactivity : Human

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