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ENY2 antibody [GT245]

Anti-ENY2 antibody [GT245] used in Western Blot (WB). GTX629542
Anti-ENY2 antibody [GT245] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629542
Anti-ENY2 antibody [GT245] used in Western Blot (WB). GTX629542
Anti-ENY2 antibody [GT245] used in IHC (Paraffin sections) (IHC-P). GTX629542

Cat. No. GTX629542

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT245

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all ENY2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Predict Reactivity

Mouse, Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ENY2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2721898

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ENY2 transcription and export complex 2 subunit , DC6 , Sus1 , e(y)2

Cellular Localization

Nucleus

Background

Component of the transcription regulatory histone acetylation (HAT) complex SAGA, a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates to a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation. May also participate in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery.

Database

Research Area

DATA IMAGES

Anti-ENY2 antibody [GT245] used in Western Blot (WB). GTX629542

GTX629542 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ENY2 antibody [GT245] (GTX629542) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ENY2 antibody [GT245] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629542

GTX629542 ICC/IF Image

ENY2 antibody detects ENY2 protein at Nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: ENY2 protein stained by ENY2 antibody (GTX629542) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ENY2 antibody [GT245] used in Western Blot (WB). GTX629542

GTX629542 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with ENY2 antibody [GT245] (GTX629542) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ENY2 antibody [GT245] used in IHC (Paraffin sections) (IHC-P). GTX629542

GTX629542 IHC-P Image

ENY2 antibody [GT245] detects ENY2 protein at nucleus in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
ENY2 antibody [GT245] (GTX629542) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Ng PK et al. Cancer Cell 2018; 33 (3) : 450-462.e10 Systematic Functional Annotation of Somatic Mutations in Cancer.
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations