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IFIT1 antibody [N1C1]

Orthogonal
Anti-IFIT1 antibody [N1C1] used in Western Blot (WB). GTX118713

Cat. No. GTX118713

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IFIT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

HeLa , HeLa(10 ng/ml hIFN-gamma1 treatment for 16hr)

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IFIT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10725875

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon induced protein with tetratricopeptide repeats 1 , C56 , G10P1 , IFI-56 , IFI-56K , IFI56 , IFIT-1 , IFNAI1 , ISG56 , P56 , RNM561

Database

Research Area

DATA IMAGES

Anti-IFIT1 antibody [N1C1] used in Western Blot (WB). GTX118713

GTX118713 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IFIT1 antibody [N1C1] (GTX118713) diluted at 1:1000.

REFERENCE

Application Reference

T Karasawa et al. Ren Fail 2023; 45 (1) : 2224890 Expression of interferon-stimulated gene 20 (ISG20), an antiviral effector protein, in glomerular endothelial cells: possible involvement of ISG20 in lupus nephritis
Application : WB
Reactivity : Human
Imaizumi T et al. Kidney Blood Press Res 2021; 46 (1) : 74-83 IFIT Proteins Are Involved in CXCL10 Expression in Human Glomerular Endothelial Cells Treated with a Toll-Like Receptor 3 Agonist.
Application : WB
Reactivity : Human
Yap GLR et al. Cell Death Dis 2020; 11 (6) : 463 Annexin-A1 promotes RIG-I-dependent signaling and apoptosis via regulation of the IRF3-IFNAR-STAT1-IFIT1 pathway in A549 lung epithelial cells.
Application : WB
Reactivity : Human
Amador-Canizares Y et al. Nucleic Acids Res 2018; (Epub) miR-122 does not impact recognition of the HCV genome by innate sensors of RNA but rather protects the 5' end from the cellular pyrophosphatases, DOM3Z and DUSP11.
Application : WB
Reactivity : Human
Antzin-Anduetza I et al. Retrovirology 2017; 14 (1) : 49 Increasing the CpG dinucleotide abundance in the HIV-1 genomic RNA inhibits viral replication.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169