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POLE antibody

Orthogonal
Anti-POLE antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132100
Anti-POLE antibody used in IHC (Paraffin sections) (IHC-P). GTX132100
Anti-POLE antibody used in Western Blot (WB). GTX132100
Anti-POLE antibody used in Western Blot (WB). GTX132100
Anti-POLE antibody used in Western Blot (WB). GTX132100
Anti-POLE antibody used in Western Blot (WB). GTX132100
Anti-POLE antibody used in Western Blot (WB). GTX132100

Cat. No. GTX132100

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all POLE products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2500
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

262 kDa. ( Note )

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human POLE. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886567

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA polymerase epsilon, catalytic subunit , CRCS12 , FILS , IMAGEI , POLE1

Cellular Localization

Nucleus

Background

Participates in DNA repair and in chromosomal DNA replication.

Database

Research Area

DATA IMAGES

Anti-POLE antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132100

GTX132100 ICC/IF Image

POLE antibody detects POLE protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: POLE protein stained by POLE antibody (GTX132100) diluted at 1:2500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Anti-POLE antibody used in IHC (Paraffin sections) (IHC-P). GTX132100

GTX132100 IHC-P Image

POLE antibody detects POLE protein at nucleus in human ovary by immunohistochemical analysis.
Sample: Paraffin-embedded human ovary.
POLE antibody (GTX132100) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-POLE antibody used in Western Blot (WB). GTX132100

GTX132100 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with POLE antibody (GTX132100) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-POLE antibody used in Western Blot (WB). GTX132100

GTX132100 WB Image

Orthogonal

The data was published in the Mol Cell in 2018. PMID: 29754823

Anti-POLE antibody used in Western Blot (WB). GTX132100

GTX132100 WB Image

Orthogonal

The data was published in the Mol Cell in 2018. PMID: 29754823

Anti-POLE antibody used in Western Blot (WB). GTX132100

GTX132100 WB Image

The data was published in the journal Mol Cell in 2018.PMID: 30217558

Anti-POLE antibody used in Western Blot (WB). GTX132100

GTX132100 WB Image

The data was published in the journal Mol Cell in 2018.PMID: 30217558

REFERENCE

Application Reference

Nakano T et al. J Med Genet 2022; Novel POLE mutations identified in patients with IMAGE-I syndrome cause aberrant subcellular localisation and protein degradation in the nucleus.
Application : WB
Reactivity : Human
Borel V et al. Cell Rep 2022; 39 (9) : 110871 Disrupted control of origin activation compromises genome integrity upon destabilization of Polε and dysfunction of the TRP53-CDKN1A/P21 axis.
Sviderskiy VO et al. Mol Cell 2020; 80 (4) : 682-698.e7 Hyperactive CDK2 Activity in Basal-like Breast Cancer Imposes a Genome Integrity Liability that Can Be Exploited by Targeting DNA Polymerase ε.
Application : WB
Reactivity : Human
Bellelli R et al. Mol Cell 2018; 70 (4) : 707-721.e7 Polε Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis.
Bellelli R et al. Mol Cell 2018; 72 (1) : 112-126.e5 POLE3-POLE4 Is a Histone H3-H4 Chaperone that Maintains Chromatin Integrity during DNA Replication.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169