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OTX2 antibody

Anti-OTX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133210
Anti-OTX2 antibody used in Western Blot (WB). GTX133210
Anti-OTX2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133210

Cat. No. GTX133210

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all OTX2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

Y79

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse OTX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886868

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

orthodenticle homeobox 2 , E130306E05Rik

Database

Research Area

DATA IMAGES

Anti-OTX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133210

GTX133210 IHC-P Image

OTX2 antibody detects OTX2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: OTX2 stained by OTX2 antibody (GTX133210) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-OTX2 antibody used in Western Blot (WB). GTX133210

GTX133210 WB Image

Whole cell extract (37.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with OTX2 antibody (GTX133210) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.

Anti-OTX2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133210

GTX133210 IHC-Fr Image

OTX2 antibody detects OTX2 protein by immunohistochemical analysis.
Sample: Frozen-sectioned rat E13.5 brain.
Green: OTX2 stained by OTX2 antibody (GTX133210) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

LeBlanc L et al. iScience 2022; 25 (1) : 103541 β-catenin links cell seeding density to global gene expression during mouse embryonic stem cell differentiation.
Pajtler KW et al. Acta Neuropathol 2018; (Epub) Molecular heterogeneity and CXorf67 alterations in posterior fossa group A (PFA) ependymomas.
Application : WB, IHC-P
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169