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FTO antibody

Anti-FTO antibody used in Western Blot (WB). GTX85019
Anti-FTO antibody used in IHC (Paraffin sections) (IHC-P). GTX85019
Anti-FTO antibody used in IHC (Paraffin sections) (IHC-P). GTX85019

Cat. No. GTX85019

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($409)
See all FTO products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ICC/IF Assay dependent
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

Human Uterus Tissue Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

FTO antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human FTO.The immunogen is located within the first 50 amino acids of FTO.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10726145

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FTO alpha-ketoglutarate dependent dioxygenase , ALKBH9 , BMIQ14 , GDFD

Cellular Localization

Nucleus,Nucleus speckle,Cytoplasm

Background

Rising obesity rates are rapidly becoming a growing health concern in the developing world. The fat mass and obesity associated gene (FTO) is the first gene discovered to contribute to common forms of human obesity. FTO is a member of the non-heme dioxygenase superfamily, encoding a 2-oxoglutarate-dependent nucleic acid demethylase whose mRNA is widely expressed, especially in neurons of feeding-related nuclei of the brain. FTO mRNA in the arcuate nucleus in mice is up-regulated by feeding and down-regulated during fasting, although the opposite pattern has been observed in rats. At least four isoforms of FTO are known to exist.

Database

Research Area

DATA IMAGES

Anti-FTO antibody used in Western Blot (WB). GTX85019

GTX85019 WB Image

WB analysis of human uterus tissue lysate using GTX85019 FTO antibody.
Working concentration : 1 μg/ml

Anti-FTO antibody used in IHC (Paraffin sections) (IHC-P). GTX85019

GTX85019 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX85019 FTO antibody.
Working concentration : 2.5 μg/ml

Anti-FTO antibody used in IHC (Paraffin sections) (IHC-P). GTX85019

GTX85019 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX85019 FTO antibody.
Working concentration : 20 μg/ml

REFERENCE

Application Reference

Bai L et al. Toxicol Sci 2018; (Epub) m6A demethylase FTO regulates dopaminergic neurotransmission deficits caused by arsenite.
Application : WB, ICC/IF
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409