Periostin antibody

Name: Periostin antibody
Brand: GeneTex
SKU: GTX100602
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Periostin antibody used in Western Blot (WB). GTX100602

Anti-Periostin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100602

Anti-Periostin antibody used in IHC (Paraffin sections) (IHC-P). GTX100602

Cat. No. GTX100602

Reference 8

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all Periostin products

View product citations for antibody GTX100602 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	Assay dependent

Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Periostin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951327

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

periostin , OSF-2 , OSF2 , PDLPOSTN , PN

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization.

Database

Gene ID: 10631 POSTN
UniProt: Q15063 POSTN

Research Area

DATA IMAGES

GTX100602 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Periostin antibody (GTX100602) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100602 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Periostin(GTX100602) antibody at 1:200 dilution.

GTX100602 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Periostin antibody (GTX100602) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX100602 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric tissue, using Periostin(GTX100602) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100602 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX100602 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100602 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Periostin antibody (GTX100602) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.