GeneTex

  • Country / Location Selection

United States (US)

p63 antibody [N2C1], Internal

KO/KD Orthogonal
Anti-p63 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102425
Anti-p63 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102425
Anti-p63 antibody [N2C1], Internal used in Western Blot (WB). GTX102425
Anti-p63 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102425
Anti-p63 antibody [N2C1], Internal used in Western Blot (WB). GTX102425
Anti-p63 antibody [N2C1], Internal used in Western Blot (WB). GTX102425
Anti-p63 antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX102425
Anti-p63 antibody [N2C1], Internal used in Western Blot (WB). GTX102425
Anti-p63 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102425
Anti-p63 antibody [N2C1], Internal used in Western Blot (WB). GTX102425
Anti-p63 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102425

Cat. No. GTX102425

Reference 57
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, PLA

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all p63 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
PLA Assay dependent
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

mouse brain , rat brain , A431

Predict Reactivity

Zebrafish, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p63. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952344

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor protein p63 , AIS , B(p51A) , B(p51B) , EEC3 , KET , LMS , NBP , OFC8 , RHS , SHFM4 , TP53CP , TP53L , TP73L , p40 , p51 , p53CP , p63 , p73H , p73L

Cellular Localization

Nucleus

Background

This gene encodes a member of the p53 family of transcription factors. An animal model, p63 -/- mice, has been useful in defining the role this protein plays in the development and maintenance of stratified epithelial tissues. p63 -/- mice have several developmental defects which include the lack of limbs and other tissues, such as teeth and mammary glands, which develop as a result of interactions between mesenchyme and epithelium. Mutations in this gene are associated with ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3); split-hand/foot malformation 4 (SHFM4); ankyloblepharon-ectodermal defects-cleft lip/palate; ADULT syndrome (acro-dermato-ungual-lacrimal-tooth); limb-mammary syndrome; Rap-Hodgkin syndrome (RHS); and orofacial cleft 8. Both alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different proteins. Many transcripts encoding different proteins have been reported but the biological validity and the full-length nature of these variants have not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-p63 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102425

GTX102425 IHC-P Image

Immunohistochemical characterization of Synaptophysin (GTX100865), p63 (GTX102425) and Cytokeratin 7 (GTX109723) in human small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) specimens.
Sample: Paraffin-embedded human SCLC (upper panel) and NSCLC (lower panel).
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 15 mins. The section was then incubated with primary antibody at 1:500 overnight at 4ºC and detected using an HRP conjugated avidin-biotin-peroxidase Complex system. DAB was used as the chromogen and counterstained with haematoxylin.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p63 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102425

GTX102425 IHC-P Image

p63 antibody [N2C1], Internal detects p63 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tongue.
p63 stained by p63 antibody [N2C1], Internal (GTX102425) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p63 antibody [N2C1], Internal used in Western Blot (WB). GTX102425

GTX102425 WB Image

p63 antibody [N2C1], Internal detects TP63 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
7.5% SDS-PAGE
p63 antibody [N2C1], Internal (GTX102425) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p63 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102425

GTX102425 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using p63(GTX102425) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-p63 antibody [N2C1], Internal used in Western Blot (WB). GTX102425

GTX102425 WB Image

KO/KD

Non-transfected (–) and transfected (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [N2C1], Internal (GTX102425) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p63 antibody [N2C1], Internal used in Western Blot (WB). GTX102425

GTX102425 WB Image

p63 antibody [N2C1], Internal detects TP63 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
7.5% SDS-PAGE
p63 antibody [N2C1], Internal (GTX102425) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p63 antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX102425

GTX102425 IP Image

Immunoprecipitation of p63 protein from A431 whole cell extracts using 5 μg of p63 antibody [N2C1], Internal (GTX102425).
Western blot analysis was performed using p63 antibody [N2C1], Internal (GTX102425).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-p63 antibody [N2C1], Internal used in Western Blot (WB). GTX102425

GTX102425 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [N2C1], Internal (GTX102425) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p63 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102425

GTX102425 ICC/IF Image

p63 antibody [N2C1], Internal detects p63 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p63 stained by p63 antibody [N2C1], Internal (GTX102425) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.

Anti-p63 antibody [N2C1], Internal used in Western Blot (WB). GTX102425

GTX102425 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2018.PMID: 30120338

Anti-p63 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102425

GTX102425 IHC-P Image

The data was published in the journal PLoS Pathog in 2018. PMID: 30543708

REFERENCE

Application Reference

Jiang Y et al. Nat Commun 2024; 15 (1) : 2484 Reciprocal inhibition between TP63 and STAT1 regulates anti-tumor immune response through interferon-γ signaling in squamous cancer.
Yamamoto S et al. Regen Ther 2023; 24 : 561-567 Successful reconstruction of the rat ureter by a syngeneic collagen tube with a cardiomyocyte sheet.
JM Lee et al. Cell Biosci 2023; 13 (1) : 108 Ahnak is required to balance calcium ion homeostasis and smooth muscle development in the urinary system
Application : IHC-P
Reactivity : Mouse
SK Russell et al. Nat Microbiol 2023; Uropathogenic Escherichia coli infection-induced epithelial trained immunity impacts urinary tract disease outcome
Application : IHC-P
Reactivity : Human
Neumayer G et al. bioRxiv 2023; A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis Bullosa.
Application : ICC/IF
Show More +
Submit a Reference

REVIEW

p63 antibody [N2C1], Internal Cat. No. GTX102425

Rating
( Average 4.8 based on 4 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 4 based on 1 users reviews)
Anti-p63 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102425
Date : Anonymous submitted on 01-Mar-2022
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : Human pancrease tissue section
Fixation : 10% neutral buffered formalin, RT, 12Hr
Permeabilization /Antigen retrieval : antigen retrieval buffer (pH 9)
Primary Antibody : 1:500, 4°C, 12Hr
Anti-p63 antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX102425
Date : Anonymous submitted on 19-Mar-2018
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : vas deferens tissue
Blocking : 10% normal goat serum + 0.025% triton-X100 in PBS, 25°C, 0.5Hr
Fixation : 4% paraformaldehyde
Permeabilization /Antigen retrieval : 0.025%triton-X100 in PBS
Primary Antibody : 1:400, 4°C, 6Hr
Anti-p63 antibody [N2C1], Internal used in Western Blot (WB). GTX102425
Date : Anonymous submitted on 21-Nov-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human TAp63gamma transfected 293T cells
Amount used : 10μg
Blocking : 2% BSA, 3% non-fat milk in PBST, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Anti-p63 antibody [N2C1], Internal used in Western Blot (WB). GTX102425
Date : Anonymous submitted on 18-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : Flag-human p63 alpha transfected Panc-1 cells
Amount used : 30μg
Blocking : 0.5% milk in TBST, 4°C, 12Hr
Primary Antibody : 1:2000, 25°C, 1Hr
Submit a Review
Application Reference
Jiang Y et al. Nat Commun 2024; 15 (1):2484 Reciprocal inhibition between TP63 and STAT1 regulates anti-tumor immune response through interferon-γ signaling in squamous cancer.
Yamamoto S et al. Regen Ther 2023; 24:561-567 Successful reconstruction of the rat ureter by a syngeneic collagen tube with a cardiomyocyte sheet.
JM Lee et al. Cell Biosci 2023; 13 (1):108 Ahnak is required to balance calcium ion homeostasis and smooth muscle development in the urinary system
Application : IHC-P
Reactivity : Mouse
SK Russell et al. Nat Microbiol 2023; Uropathogenic Escherichia coli infection-induced epithelial trained immunity impacts urinary tract disease outcome
Application : IHC-P
Reactivity : Human
Neumayer G et al. bioRxiv 2023; A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis Bullosa.
Application : ICC/IF
Chalmers FE et al. Dev Biol 2022; 485:9-23 Targeted deletion of TGFβ1 in basal keratinocytes causes profound defects in stratified squamous epithelia and aberrant melanocyte migration.
Bedos L et al. Front Vet Sci 2022; 9:1050467 Culture and characterization of canine and feline corneal epithelial organoids: A new tool for the study and treatment of corneal diseases.
Application : IHC-P
Reactivity : Cat, Dog
Huang H et al. Elife 2022; 11 Contribution of <i>Trp63<sup>CreERT2</sup></i>-labeled cells to alveolar regeneration is independent of tuft cells.
Huang H et al. Sci Adv 2022; 8 (28):eabo5668 Cis-regulatory chromatin loops analysis identifies GRHL3 as a master regulator of surface epithelium commitment.
Kadur Lakshminarasimha Murthy P et al. Nature 2022; 604 (7904):111-119 Human distal lung maps and lineage hierarchies reveal a bipotent progenitor.
Spieker AJ et al. Histopathology 2021; 79 (6):1061-1071 PTEN expression and morphological patterns in prostatic adenocarcinoma.
Xiyin Wang et al. Am J Pathol 2021; 191 (7):1281-1291 Vaginal Squamous Cell Carcinoma Develops in Mice with Conditional Arid1a Loss and Gain of Oncogenic Kras Driven by Progesterone Receptor Cre.
Application : IHC-P
Reactivity : Mouse
Gaku Takano et al. Mol Ther Oncolytics 2021; 20:220-227 Oncolytic activity of naturally attenuated herpes-simplex virus HF10 against an immunocompetent model of oral carcinoma.
Application : IHC-P
Reactivity : Mouse
Mou H et al. Cell Rep 2021; 35 (3):109011 Airway basal stem cells generate distinct subpopulations of PNECs.
Application : IHC-P
Reactivity : Human
Omote N et al. Physiol Rep 2021; 9 (3):e14727 Long noncoding RNA TINCR is a novel regulator of human bronchial epithelial cell differentiation state.
Application : WB
Reactivity : Human
Katsura H et al. Cell Stem Cell 2020; 27 (6):890-904.e8 Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction.
Application : IHC-P
Reactivity : Human
Hu L et al. PLoS One 2020; 15 (9):e0238852 Human esophageal myofibroblasts increase squamous epithelial thickness via paracrine mechanisms in an in vitro model of gastroesophageal reflux disease.
Application : IHC-P
Reactivity : Human
Kim DE et al. Aging Cell 2020; 19 (3):e13072 Deficiency in the DNA repair protein ERCC1 triggers a link between senescence and apoptosis in human fibroblasts and mouse skin.
Lu J et al. Pediatr Res 2020; Rho/SMAD/mTOR triple inhibition enables long-term expansion of human neonatal tracheal aspirate-derived airway basal cell-like cells.
Application : ICC/IF
Marsh T et al. Dev Cell 2020; 52 (5):591-604.e6 Autophagic Degradation of NBR1 Restricts Metastatic Outgrowth during Mammary Tumor Progression.
Application : IHC-P
Liu C et al. Nat Commun 2019; 10 (1):4589 Pparg promotes differentiation and regulates mitochondrial gene expression in bladder epithelial cells.
Application : ICC/IF
S.Noguchi et al. Journal of Comparative Pathology 2019; 173:30-40 Expression of Tumour Endothelial Marker 8 in Canine Mammary Gland Tumour Cells.
Application : IHC-P
Reactivity : Dog
Aljagthmi AA et al. Cell Death Dis 2019; 10 (9):680 ΔNp63α suppresses cells invasion by downregulating PKCγ/Rac1 signaling through miR-320a.
Application : WB
Reactivity : Human
Stacy AJ et al. J Biol Chem 2019; TIP60 upregulates ΔNp63α to promote cellular proliferation.
Application : WB
Reactivity : Human
Jiang Y et al. Transl Oncol 2019; 12 (7):959-967 Cytological Immunostaining of HMGA2, LRP1B, and TP63 as Potential Biomarkers for Triaging Human Papillomavirus-Positive Women.
Application : IHC-P
Reactivity : Human
Feldman MB et al. Am J Respir Cell Mol Biol 2019; SMAD Signaling Restricts Mucous Cell Differentiation In Human Airway Epithelium.
Application : ICC/IF
Reactivity : Human
Li L et al. Cell Stem Cell 2019; 24 (2):271-284.e8 TFAP2C- and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment.
Application : ICC/IF
Reactivity : Human
Fernandes-Cunha GM et al. Stem Cells Transl Med 2019; (Epub) Corneal Wound Healing Effects of Mesenchymal Stem Cell Secretome Delivered Within a Viscoelastic Gel Carrier.
Application : ICC/IF
Reactivity : Human
Levardon H et al. Bio Protoc 2018; 8 (11) Expansion of Airway Basal Cells and Generation of Polarized Epithelium.
Wang J et al. Cell Rep 2018; 25 (2):464-477.e4 Polyploid Superficial Cells that Maintain the Urothelial Barrier Are Produced via Incomplete Cytokinesis and Endoreplication.
Pattison JM et al. Nat Genet 2018; 50 (12):1658-1665 Retinoic acid and BMP4 cooperate with p63 to alter chromatin dynamics during surface epithelial commitment.
O'Brien VP et al. PLoS Pathog 2018; 14 (12):e1007457 Host restriction of Escherichia coli recurrent urinary tract infection occurs in a bacterial strain-specific manner.
Lee J et al. J Biomed Mater Res B Appl Biomater 2018; (Epub) Treatment of intractable oral ulceration with an oral mucosa equivalent.
Application : IHC-P
Reactivity : Rat
Jillian M. Pattison et al. Nature Genetics 2018; (Epub) Retinoic acid and BMP4 cooperate with p63 to alter chromatin dynamics during surface epithelial commitment.
Application : ICC/IF
Reactivity : Human
Li HD et al. J Clin Invest 2018; 128 (9):4179-4191 Polymerase-mediated ultramutagenesis in mice produces diverse cancers with high mutational load.
Application : IHC-P
Reactivity : Mouse
Zhang C et al. Sci Rep 2018; 8 (1):12354 Human esophageal myofibroblast secretion of bone morphogenetic proteins and GREMLIN1 and paracrine regulation of squamous epithelial growth.
Application : WB
Montoro DT et al. Nature 2018; 560 (7718):319-324 A revised airway epithelial hierarchy includes CFTR-expressing ionocytes.
Application : ICC/IF
Reactivity : Mouse
Nishiyama K et al. Cell Death Dis 2018; 9 (8):826 Screening for long noncoding RNAs associated with oral squamous cell carcinoma reveals the potentially oncogenic actions of DLEU1.
Application : WB
Reactivity : Human
Sandra P Melo et al. bioRxiv 2018; (Epub) Morphogen-Lineage Selector Interactions During Surface Epithelial Commitment.
Application : ICC/IF
Reactivity : Human
Sakaram S et al. Sci Rep 2018; 8 (1):10069 Identification of novel ΔNp63α-regulated miRNAs using an optimized small RNA-Seq analysis pipeline.
Application : WB
Reactivity : Human
Nguyen QH et al. Nat Commun 2018; 9 (1):2028 Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity.
Application : IHC-P
Reactivity : Human
Sang M et al. Oncol Rep 2018; (Epub) impact of runx2 gene silencing on the * sensitivity of p53-mutated pancreatic cancer miapaca-2 spheres.
Tata PR et al. Dev Cell 2018; 44 (6):679-693.e5 Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity.
Application : IHC-Fr
Reactivity : Mouse
Tata A et al. Cell Stem Cell 2018; (Epub) Myoepithelial Cells of Submucosal Glands Can Function as Reserve Stem Cells to Regenerate Airways after Injury.
Tamai R et al. Can J Microbiol 2018; (Epub) Extracellular galectin-1 enhances adhesion to and invasion of oral epithelial cells by Porphyromonas gingivalis.
Application : IHC-P
Sch?fer FM et al. Stem Cell Reports 2017; 9 (6):2005-2017 Mode of Surgical Injury Influences the Source of Urothelial Progenitors during Bladder Defect Repair.
Roh JL et al. Oral Oncology 2017; 75 (2017):81-88 Plasticity of oral mucosal cell sheets for accelerated and scarless skin wound healing.
Application : IHC-Fr
Reactivity : Human
He K et al. Sci Rep 2017; 7 (1):13685 Myosin X is recruited to nascent focal adhesions at the leading edge and induces multi-cycle filopodial elongation.
Application : IHC-P
Reactivity : Human
Xiong Z et al. J Pathol 2017; (Epub) PAX9 regulates squamous cell differentiation and carcinogenesis in the oro-esophageal epithelium.
Application : IHC-P
Reactivity : Mouse
Jin H et al. J Biol Chem 2017; (Epub) p63α protein upregulates heat shock protein 70 expression via E2F1 transcription factor 1, promoting Wasf3/Wave3/MMP9 signaling and bladder cancer invasion.
Application : WB
Reactivity : Human
Kim BR et al. Mol Cell Proteomics 2017; (Epub) Identification of the SOX2 interactome by BioID reveals EP300 as a mediator of SOX2-dependent squamous differentiation and lung squamous cell carcinoma growth.
Application : PLA
Reactivity : Human
Reneker LW et al. Invest Ophthalmol Vis Sci 2017; 58 (5):2638-2646 Fibroblast Growth Factor Receptor 2 (FGFR2) Is Required for Meibomian Gland Homeostasis in the Adult Mouse.
Application : IHC-P
Reactivity : Mouse
O'Brien VP et al. Nat Microbiol 2016; 2:16196 A mucosal imprint left by prior Escherichia coli bladder infection sensitizes to recurrent disease.
Application : IHC-P
Reactivity : Rat
Sasaki Y et al. Oncotarget 2016; (Epub) Identification and characterization of the intercellular adhesion molecule-2 gene as a novel p53 target.
Application : WB
Reactivity : Human
Qu Y et al. Sci Rep 2016; 6:32007 Transcriptome and proteome characterization of surface ectoderm cells differentiated from human iPSCs.
Application : WB, ICC/IF
Reactivity : Human
Zhu D et al. Int J Gynecol Cancer 2014; Amplification and Overexpression of TP63 and MYC as Biomarkers for Transition of Cervical Intraepithelial Neoplasia to Cervical Cancer.
Chang HL et al. Biochim Biophys Acta 2013; 1830 (8):4053-64 Simvastatin induced HCT116 colorectal cancer cell apoptosis through p38MAPK-p53-survivin signaling cascade.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169