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ALDH1A1 antibody

Orthogonal
Anti-ALDH1A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX123973
Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973
Anti-ALDH1A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX123973
Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973
Anti-ALDH1A1 antibody used in IHC (Whole mount) (IHC-Wm). GTX123973
Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973
Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973
Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973
Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973
Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973
Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973

Cat. No. GTX123973

Reference 24
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish, Cat
Package
100 μl ($399),
25 μl ($169)
See all ALDH1A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

A549 , mouse liver , rat liver

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH1A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166810

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldehyde dehydrogenase 1 family member A1 , ALDC , ALDH-E1 , ALDH1 , ALDH11 , HEL-9 , HEL-S-53e , HEL12 , PUMB1 , RALDH1

Cellular Localization

Cytoplasm

Background

This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes. [provided by RefSeq]

Database

  • Gene ID: 216 ALDH1A1
  • UniProt: P00352 ALDH1A1

Research Area

DATA IMAGES

Anti-ALDH1A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX123973

GTX123973 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using ALDH1A1(GTX123973) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973

GTX123973 WB Image

ALDH1A1 antibody detects ALDH1A1 protein by western blot analysis.
A. 50 μg rat liver lysate/extract
10% SDS-PAGE
ALDH1A1 antibody (GTX123973) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALDH1A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX123973

GTX123973 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using ALDH1A1(GTX123973) antibody at 1:500 dilution.

Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973

GTX123973 WB Image

ALDH1A1 antibody detects ALDH1A1 protein by western blot analysis.
A. 50 μg mouse liver lysate/extract
10% SDS-PAGE
ALDH1A1 antibody (GTX123973) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALDH1A1 antibody used in IHC (Whole mount) (IHC-Wm). GTX123973

GTX123973 IHC-Wm Image

ALDH1A1 antibody detects Aldh1a2 protein on whole-mount zebrafish embryos by immunohistochemical analysis.?
Sample: Paraformaldehyde-fixed zebrafish embryos.
ALDH1A1 antibody (GTX123973) dilution: 1:200.

Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973

GTX123973 WB Image

PG-4 whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A1 antibody (GTX123973) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973

GTX123973 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A1 antibody (GTX123973) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973

GTX123973 WB Image

Orthogonal

The data was published in the journal Mol Oncol in 2020.PMID: 32037723

Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973

GTX123973 WB Image

Orthogonal

The data was published in the 2021 in Cancer Drug Resist. PMID: 35582384

Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973

GTX123973 WB Image

The data was published in the journal Cell Death Dis in 2015. PMID: 26136074

Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973

GTX123973 WB Image

The data was published in the 2020 in Front Pharmacol. PMID: 33381038

REFERENCE

Application Reference

TY Chang et al. Int J Biol Sci 2024; 20 (3) : 880–896 Nε-(1-Carboxymethyl)-L-lysine/RAGE Signaling Drives Metastasis and Cancer Stemness through ERK/NFκB axis in Osteosarcoma
BY Wang et al. Am J Cancer Res 2023; 13 (10) : 4721-4733 Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression
Martinez PA et al. Brain Behav 2023; 13 (9) : e3150 Impaired aldehyde detoxification exacerbates motor deficits in an alpha-synuclein mouse model of Parkinson's disease.
Guo S et al. Cell Mol Life Sci 2023; 80 (9) : 270 TRPM7 transactivates the FOSL1 gene through STAT3 and enhances glioma stemness.
Masato A et al. NPJ Parkinsons Dis 2023; 9 (1) : 42 DOPAL initiates αSynuclein-dependent impaired proteostasis and degeneration of neuronal projections in Parkinson's disease.
Application : WB, ICC/IF
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

ALDH1A1 antibody Cat. No. GTX123973

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973
Date : Anonymous submitted on 18-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : T24 cells
Amount used : 1* 10^5 cells
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973
Date : Anonymous submitted on 24-Dec-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells, HepG2 cells
Amount used : 20μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 16Hr
Lane Description : Lane A: HepG2 cells; Lane B: Huh7 cells
Submit a Review
Application Reference
TY Chang et al. Int J Biol Sci 2024; 20 (3):880–896 Nε-(1-Carboxymethyl)-L-lysine/RAGE Signaling Drives Metastasis and Cancer Stemness through ERK/NFκB axis in Osteosarcoma
BY Wang et al. Am J Cancer Res 2023; 13 (10):4721-4733 Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression
Martinez PA et al. Brain Behav 2023; 13 (9):e3150 Impaired aldehyde detoxification exacerbates motor deficits in an alpha-synuclein mouse model of Parkinson's disease.
Guo S et al. Cell Mol Life Sci 2023; 80 (9):270 TRPM7 transactivates the FOSL1 gene through STAT3 and enhances glioma stemness.
Masato A et al. NPJ Parkinsons Dis 2023; 9 (1):42 DOPAL initiates αSynuclein-dependent impaired proteostasis and degeneration of neuronal projections in Parkinson's disease.
Application : WB, ICC/IF
Reactivity : Mouse
Seung-Hun Kim et al. Cancer Drug Resist 2021; 4 (4):866-880 The aqueous extract of brucea javanica reduces tumorigenicity of human lung cancer tumorspheres.
Application : WB
Reactivity : Human
Takahashi H et al. Stem Cell Res Ther 2021; 12 (1):471 Antifibrotic effect of lung-resident progenitor cells with high aldehyde dehydrogenase activity.
Application : IHC-P
Reactivity : Mouse
King P et al. Int J Oncol 2021; 59 (4) Regulation of gliomagenesis and stemness through acid sensor ASIC1a.
Reactivity : Human
Morello G et al. Cancer Immunol Res 2021; 9 (7):825-837 T Cells Expressing Receptor Recombination/Revision Machinery Are Detected in the Tumor Microenvironment and Expanded in Genomically Over-unstable Models.
Application : IHC-P
Reactivity : Mouse
Talaat S.M. et al. Learning Materials in Biosciences 2021; (Epub) Immunohistochemical expression of stem cell markers ALDH1 and CD44 in urothelial carcinoma of the urinary bladder.
Jingwei Wan et al. Front Pharmacol 2020; 11:590723 TRPM7 Induces Tumorigenesis and Stemness Through Notch Activation in Glioma.
Application : WB
Reactivity : Human
Zhang J et al. Int J Mol Sci 2020; 21 (9) ALDH1A1 Inhibits Chicken Preadipocytes' Proliferation and Differentiation via the PPARγ Pathway In Vitro and In Vivo.
Lai HT et al. Mol Oncol 2020; 14 (6):1327-1347 GATA6 enhances the stemness of human colon cancer cells by creating a metabolic symbiosis through upregulating LRH-1 expression.
Lin YY et al. Int J Med Sci 2020; 17 (11):1639-1651 PODXL2 maintains cellular stemness and promotes breast cancer development through the Rac1/Akt pathway.
Application : WB
Reactivity : Human
Kitakaze T et al. Arch Biochem Biophys 2020; 686:108329 6-(Methylsulfinyl)hexyl isothiocyanate protects acetaldehyde-caused cytotoxicity through the induction of aldehyde dehydrogenase in hepatocytes.
Application : WB
Reactivity : Mouse
Fletcher PA et al. Front Endocrinol (Lausanne) 2019; 10:623 Cell Type- and Sex-Dependent Transcriptome Profiles of Rat Anterior Pituitary Cells.
Application : ICC/IF
Reactivity : Rat
Castagnoli L et al. Oncogene 2019; (Epub) WNT signaling modulates PD-L1 expression in the stem cell compartment of triple-negative breast cancer.
Reactivity : Human
Chen L et al. J Cell Mol Med 2017; (Epub) TRAF6 regulates tumour metastasis through EMT and CSC phenotypes in head and neck squamous cell carcinoma.
Application : WB
Reactivity : Human
Ma L et al. PLoS One 2017; 12 (11):e0188665 Suppression of cancer stemness by upregulating Ligand-of-Numb protein X1 in colorectal carcinoma.
Application : WB
Reactivity : Human
Huang CF et al. J Exp Clin Cancer Res 2017; 36 (1):116 NLRP3 inflammasome activation promotes inflammation-induced carcinogenesis in head and neck squamous cell carcinoma.
Application : WB
Reactivity : Mouse
Ni YL et al. Apoptosis 2017; (Epub) A potent indolylquinoline alleviates growth of human lung cancer cell tumorspheres.
Application : WB
Reactivity : Human
Wu TF et al. Tumour Biol 2017; 39 (4):1.01E+15 Expression and associations of TRAF1, BMI-1, ALDH1, and Lin28B in oral squamous cell carcinoma.
Application : IHC-P
Reactivity : Human
Yan L et al. Sci Rep 2016; 6:28281 MiR-21-5p Links Epithelial-Mesenchymal Transition Phenotype with Stem-Like Cell Signatures via AKT Signaling in Keloid Keratinocytes.
Application : WB
Reactivity : Human
Ju SY et al. Cell Death Dis 2015; 6 (Epub):e1801 identification of * as a novel therapeutic agent that targets human colon cancer stem cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169