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nNOS antibody

Anti-nNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX82905
Anti-nNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX82905
Anti-nNOS antibody used in Western Blot (WB). GTX82905
Anti-nNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82905
Anti-nNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX82905

Cat. No. GTX82905

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

 Human, Mouse, Rat, Zebrafish, Rabbit, Bovine, Guinea pig
Package
100 μl ($399)
See all nNOS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:250
IHC-P 1:200-400
IHC-Fr 1:800
IP Assay dependent
Not tested in other applications.

Calculated MW

160 kDa. ( Note )

Product Note

This antibody does not detect endothelial NOS (eNOS) or inducible NOS (iNOS).

PROPERTIES

Form

Liquid

Buffer

Serum diluted with PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

Synthetic peptide corresponding to residues T(724) K R R A I G F K K L A E A V K(739) C of rat bNOS.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_625814

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nitric oxide synthase 1, neuronal , 2310005C01Rik , N-NOS , NC-NOS , NO , NOS , NOS-I , Nos-1 , bNOS , nNOS

Background

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. eNOS and bNOS share ~50% sequence homology, and their activity depends on binding to the calcium/calmodulin complex. Both constitutive isoforms respond immediately to increased levels of calcium to produce low levels of NO over a short period of time. bNOS has been found in neurons, peripheral nerve cells, macula densa & pancreatic islet cells.

Database

Research Area

DATA IMAGES

Anti-nNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX82905

GTX82905 IHC-P Image

IHC-P analysis of rat cerebellum tissue using GTX82905 nNOS antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-nNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX82905

GTX82905 IHC-P Image

IHC-P analysis of mouse skeletal muscle tissue using GTX82905 nNOS antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:400

Anti-nNOS antibody used in Western Blot (WB). GTX82905

GTX82905 WB Image

WB analysis of 40 μg of mouse (Lane 1) and rat brain (Lane 2) tissue lysate using GTX82905 nNOS antibody.
Dilution : 1:1000

Anti-nNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82905

GTX82905 ICC/IF Image

ICC/IF analysis of SH-SY5Y cells using GTX82905 nNOS antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

Anti-nNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX82905

GTX82905 IHC-P Image

IHC-P analysis of rat brain tissue using GTX82905 nNOS antibody.

REFERENCE

Application Reference

Chou CC et al. J Cardiovasc Pharmacol 2020; 75 (1) : 64-74 Single Bolus Rosuvastatin Accelerates Calcium Uptake and Attenuates Conduction Inhomogeneity in Failing Rabbit Hearts With Regional Ischemia-Reperfusion Injury.
Application : WB
Reactivity : Rabbit
Demirpence S et al. Curr Ther Res Clin Exp 2009; 70 (2) : 129-35 The effect of * on nitric oxide synthase enzyme production after iatrogenic inflammation in the brain stem of adolescent rats: A randomized, controlled, experimental study.
Application : IHC-P
Reactivity : Rat
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399