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Summary It usually blocks the antibody activity completely in immunohistochemistry analysis by pre-incubating the antibody with a 1-10X molar volume of peptide for 60 minutes at room temperature. Optimization of the molar ratio of antigen to antibody may be required.

Summary This antibody is suitable for use as a negative control to assess non-specific binding of mouse IgG2a antibodies to rat cells. And It is not suitable for use as a negative control on human.

Summary This antibody is suitable for use as a negative control to assess non-specific binding of mouse IgG1 antibodies to target cells. And It is not suitable for use as a negative control on human.