APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| Assay dependent |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
MDCK
Predict Reactivity
Human, Rabbit, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.
Concentration
0.38 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Recombinant protein corresponding to dog Albumin.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
albumin,CSA
Cellular Localization
Secreted
Background
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
Database
Research Area
DATA IMAGES
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GTX134897 WB Image
PG-4 whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (GTX134897) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX134897 IHC-P Image
Albumin antibody detects Albumin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog liver.Albumin stained by Albumin antibody (GTX134897) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX134897 IHC-P Image
Albumin antibody detects secreted Albumin protein by immunohistochemical analysis.Sample: Paraffin-embedded dog kidney.Albumin stained by Albumin antibody (GTX134897) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX134897 IHC-P Image
Albumin antibody detects Albumin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded cat liver.Albumin stained by Albumin antibody (GTX134897) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX134897 ICC/IF Image
Albumin antibody detects Albumin protein at cytoplasm by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Albumin stained by Albumin antibody (GTX134897) diluted at 1:500.Blue: Hoechst 33342 staining.
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GTX134897 ICC/IF Image
Albumin antibody detects Albumin protein at cytoplasm by immunofluorescent analysis.Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Albumin stained by Albumin antibody (GTX134897) diluted at 1:500.Blue: Hoechst 33342 staining.
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GTX134897 WB Image
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (GTX134897) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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REFERENCE
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REVIEW
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