APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| Assay dependent |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
MDCK
Predict Reactivity
Human, Rabbit, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.
Concentration
0.38 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Recombinant protein corresponding to dog Albumin.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
albumin , CSA
Cellular Localization
Secreted
Background
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
Database
Research Area
DATA IMAGES
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GTX134897 WB Image
PG-4 whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (GTX134897) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX134897 IHC-P Image
Albumin antibody detects Albumin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog liver.Albumin stained by Albumin antibody (GTX134897) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX134897 IHC-P Image
Albumin antibody detects secreted Albumin protein by immunohistochemical analysis.Sample: Paraffin-embedded dog kidney.Albumin stained by Albumin antibody (GTX134897) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX134897 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (GTX134897) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX134897 IHC-P Image
Albumin antibody detects Albumin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded cat liver.Albumin stained by Albumin antibody (GTX134897) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX134897 ICC/IF Image
Albumin antibody detects Albumin protein at cytoplasm by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Albumin stained by Albumin antibody (GTX134897) diluted at 1:500.Blue: Hoechst 33342 staining.
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GTX134897 ICC/IF Image
Albumin antibody detects Albumin protein at cytoplasm by immunofluorescent analysis.Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Albumin stained by Albumin antibody (GTX134897) diluted at 1:500.Blue: Hoechst 33342 staining.
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REFERENCE
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REVIEW
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