GeneTex
United States (US)

BRCC36 antibody

GTX31248_148_IHC-P_18121410_336.jpg
GTX31248_149_WB_18121410_640.jpg

Cat No. GTX31248

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1μg/ml
IHC-P Assay dependent

Note :

WB
This antibody detects a band of approximately 36kDa in MCF7 cell lysates.

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Specificity/Sensitivity

This antibody recognises human BRCA1/BRCA2 complex subunit 36 (BRCC36), a 36.7 kDa nuclear protein.
PROPERTIES

Form

Liquid

Buffer

PBS, 0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

An 18 amino acid peptide from near the amino terminus of human BRCC36.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Brca1/Brca2-Containing Complex Subunit 3,Brcc36,C6.1A,Cxorf53,Brcc3

Background

This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES
GTX31248_148_IHC-P_18121410_336.jpg

GTX31248 IHC-P Image

IHC-P analysis of human brain tissue using GTX31248 BRCC36 antibody.

GTX31248_149_WB_18121410_640.jpg

GTX31248 WB Image

WB analysis of MCF-7 cell lysate using GTX31248 BRCC36 antibody at (A)0.5, (B)1 and (C)2 ug/ml.

Package List Price ($)
$ 329