PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
|
Not tested in other applications.
Calculated MW
Positive Control
Kidney: All cells should display an at least moderate nuclear SMARCA4 immunostaining.
Negative Control
A tumor (or cell line) with documented SMARCA4 expression loss should be used as a “negative” control (as SMARCA4 is expressed in all normal tissues)
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (around aa 190-400) of human SMARCA4 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 , BAF190 , BAF190A , BRG1 , CSS4 , MRD16 , RTPS2 , SNF2 , SNF2L4 , SNF2LB , SWI2 , hSNF2b
Cellular Localization
Nucleus
Background
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
Database
Research Area
DATA IMAGES
![Anti-BRG1 antibody [MSVA-397R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04486](/templates/default/js/lightbox/images/loading.gif "Anti-BRG1 antibody [MSVA-397R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04486")
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GTX04486 IHC-P Image
IHC-P analysis of human pulmonary adenocarcinoma tissue using GTX04486 BRG1 antibody [MSVA-397R] HistoMAX™.
Pulmonary adenocarcinoma with complete loss of BRG1 staining of tumor cells.
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GTX04486 IHC-P Image
IHC-P analysis of human endometrium tissue using GTX04486 BRG1 antibody [MSVA-397R] HistoMAX™.
Intense nuclear BRG1 staining of all cell types of the endometrium.
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GTX04486 IHC-P Image
IHC-P analysis of human pancreatic ductal adenocarcinoma (PDAC) tissue using GTX04486 BRG1 antibody [MSVA-397R] HistoMAX™.
Ductal adenocarcinoma of the pancreas with complete loss of BRG1 staining in all tumor cells.
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REFERENCE
There are currently no references for BRG1 antibody [MSVA-397R] HistoMAX™ (GTX04486). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for BRG1 antibody [MSVA-397R] HistoMAX™ (GTX04486). Be the first to share your experience with this product.
![Anti-BRG1 antibody [MSVA-397R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04486](/upload/website/prouct_img/small/GTX04486/GTX04486_20230728_IHC-P_109_23072722_462.webp "Anti-BRG1 antibody [MSVA-397R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04486")
![Anti-BRG1 antibody [MSVA-397R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04486](/upload/website/prouct_img/small/GTX04486/GTX04486_20230728_IHC-P_239_23072723_405.webp "Anti-BRG1 antibody [MSVA-397R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04486")
![Anti-BRG1 antibody [MSVA-397R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04486](/upload/website/prouct_img/small/GTX04486/GTX04486_20230728_IHC-P_369_23072723_503.webp "Anti-BRG1 antibody [MSVA-397R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04486")
![Anti-BRG1 antibody [MSVA-397R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04486](/upload/website/prouct_img/normal/GTX04486/GTX04486_20230728_IHC-P_109_23072722_462.webp "Anti-BRG1 antibody [MSVA-397R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04486")
![Anti-BRG1 antibody [MSVA-397R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04486](/upload/website/prouct_img/normal/GTX04486/GTX04486_20230728_IHC-P_239_23072723_405.webp "Anti-BRG1 antibody [MSVA-397R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04486")
![Anti-BRG1 antibody [MSVA-397R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04486](/upload/website/prouct_img/normal/GTX04486/GTX04486_20230728_IHC-P_369_23072723_503.webp "Anti-BRG1 antibody [MSVA-397R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04486")