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Bmi1 antibody - VetSignal

Anti-Bmi1 antibody - VetSignal used in Western Blot (WB). GTX134944
Anti-Bmi1 antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134944
Anti-Bmi1 antibody - VetSignal used in Western Blot (WB). GTX134944
Anti-Bmi1 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134944
Anti-Bmi1 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134944

Cat No. GTX134944

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Cat, Dog
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

MDCK

Predict Reactivity

Human, Mouse, Rat, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein corresponding to dog Bmi1.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887395

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BMI1 proto-oncogene, polycomb ring finger

Database

Research Area

DATA IMAGES

Anti-Bmi1 antibody - VetSignal used in Western Blot (WB). GTX134944

GTX134944 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX134944) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bmi1 antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134944

GTX134944 IHC-P Image

Bmi1 antibody detects Bmi1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded dog lung.
Bmi1 stained by Bmi1 antibody (GTX134944) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Bmi1 antibody - VetSignal used in Western Blot (WB). GTX134944

GTX134944 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX134944) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Bmi1 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134944

GTX134944 ICC/IF Image

Bmi1 antibody detects Bmi1 protein at nucleus by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bmi1 stained by Bmi1 antibody (GTX134944) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.

Anti-Bmi1 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134944

GTX134944 ICC/IF Image

Bmi1 antibody detects Bmi1 protein at nucleus by immunofluorescent analysis.
Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bmi1 stained by Bmi1 antibody (GTX134944) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329