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Bmi1 antibody - VetSignal

Anti-Bmi1 antibody - VetSignal used in Western Blot (WB). GTX134944
Anti-Bmi1 antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134944
Anti-Bmi1 antibody - VetSignal used in Western Blot (WB). GTX134944
Anti-Bmi1 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134944
Anti-Bmi1 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134944

Cat No. GTX134944

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Cat, Dog
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

MDCK

Predict Reactivity

Human, Mouse, Rat, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein corresponding to dog Bmi1.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BMI1 proto-oncogene, polycomb ring finger

Database

Research Area

DATA IMAGES

Anti-Bmi1 antibody - VetSignal used in Western Blot (WB). GTX134944

GTX134944 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX134944) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Bmi1 antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134944

GTX134944 IHC-P Image

Bmi1 antibody detects Bmi1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded dog lung.
Bmi1 stained by Bmi1 antibody (GTX134944) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Bmi1 antibody - VetSignal used in Western Blot (WB). GTX134944

GTX134944 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX134944) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bmi1 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134944

GTX134944 ICC/IF Image

Bmi1 antibody detects Bmi1 protein at nucleus by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bmi1 stained by Bmi1 antibody (GTX134944) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.

Anti-Bmi1 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134944

GTX134944 ICC/IF Image

Bmi1 antibody detects Bmi1 protein at nucleus by immunofluorescent analysis.
Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bmi1 stained by Bmi1 antibody (GTX134944) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319