PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
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Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on the testing result, it stains almost identically for pH 6.0 to 9.0.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
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Not tested in other applications.
Calculated MW
Positive Control
A moderate to strong cytoplasmic staining of macrophages should be seen in the interfollicular zones of the tonsil, lamina propria of the appendix and in the Kupffer cells of the liver (see images). Macrophages surrounding the vessels in brain specimens should stain positive, while microglial cells should not show CD163 staining.
Negative Control
CD163 staining should not be seen in hepatocytes and in epithelial cells of the appendix and tonsil.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for BSA and azide free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human CD163 protein (around aa 43-196)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD163 molecule , M130 , MM130 , SCARI1
Cellular Localization
Cell membrane
Background
The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
Database
Research Area
DATA IMAGES
![Anti-CD163 antibody [MSVA-163M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04381](/templates/default/js/lightbox/images/loading.gif "Anti-CD163 antibody [MSVA-163M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04381")
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GTX04381 IHC-P Image
IHC-P analysis of human liver tissue section using GTX04381 CD163 antibody [MSVA-163M] HistoMAX™.
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GTX04381 IHC-P Image
IHC-P analysis of human appendix mucosa tissue section using GTX04381 CD163 antibody [MSVA-163M] HistoMAX™.
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GTX04381 IHC-P Image
IHC-P analysis of human colorectal adenocarcinoma tissue section using GTX04381 CD163 antibody [MSVA-163M] HistoMAX™.
Adenocarcinoma of the colon with low content of CD163 positive macrophages.
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REFERENCE
There are currently no references for CD163 antibody [MSVA-163M] HistoMAX™ (GTX04381). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD163 antibody [MSVA-163M] HistoMAX™ (GTX04381). Be the first to share your experience with this product.
![Anti-CD163 antibody [MSVA-163M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04381](/upload/website/prouct_img/small/GTX04381/GTX04381_20230728_IHC-P_284_23072723_654.webp "Anti-CD163 antibody [MSVA-163M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04381")
![Anti-CD163 antibody [MSVA-163M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04381](/upload/website/prouct_img/small/GTX04381/GTX04381_20230728_IHC-P_24_23072722_489.webp "Anti-CD163 antibody [MSVA-163M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04381")
![Anti-CD163 antibody [MSVA-163M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04381](/upload/website/prouct_img/small/GTX04381/GTX04381_20230728_IHC-P_154_23072722_232.webp "Anti-CD163 antibody [MSVA-163M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04381")
![Anti-CD163 antibody [MSVA-163M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04381](/upload/website/prouct_img/normal/GTX04381/GTX04381_20230728_IHC-P_284_23072723_654.webp "Anti-CD163 antibody [MSVA-163M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04381")
![Anti-CD163 antibody [MSVA-163M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04381](/upload/website/prouct_img/normal/GTX04381/GTX04381_20230728_IHC-P_24_23072722_489.webp "Anti-CD163 antibody [MSVA-163M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04381")
![Anti-CD163 antibody [MSVA-163M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04381](/upload/website/prouct_img/normal/GTX04381/GTX04381_20230728_IHC-P_154_23072722_232.webp "Anti-CD163 antibody [MSVA-163M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04381")