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CD22 antibody [MSVA-022R] HistoMAX™

Comparison HistoMAX
Anti-CD22 antibody [MSVA-022R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04393
Anti-CD22 antibody [MSVA-022R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04393
Anti-CD22 antibody [MSVA-022R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04393

Cat. No. GTX04393

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 MSVA-022R

Isotype

 IgG

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($629),
100 μl ($299)
See all CD22 products
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.

This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
Not tested in other applications.

Calculated MW

95 kDa. ( Note )

Positive Control

Tonsil or appendix: A strong, predominantly membranous CD22 staining of the germinal centre and mantle zone B-cells as well as of few interfollicular B-cells should be seen.

Negative Control

Tonsil or appendix: A large fraction of the interfollicular lymphocytes and all epithelial cells must not show any CD22 staining.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA (Please contact us for BSA and azide free format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa52-178) of human BL-CAM/CD22 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD22 molecule , SIGLEC-2 , SIGLEC2

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

Predicted to enable CD4 receptor binding activity; protein phosphatase binding activity; and sialic acid binding activity. Involved in B cell activation; negative regulation of B cell receptor signaling pathway; and regulation of endocytosis. Located in early endosome and recycling endosome. [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-CD22 antibody [MSVA-022R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04393

GTX04393 IHC-P Image

IHC-P analysis of human B-cell chronic lymphocytic leukemia tissue section using GTX04393 CD22 antibody [MSVA-022R] HistoMAX™.
B-CLL exhibiting strong CD22 staining of tumor cells.

Anti-CD22 antibody [MSVA-022R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04393

GTX04393 IHC-P Image

IHC-P analysis of human spleen tissue section using GTX04393 CD22 antibody [MSVA-022R] HistoMAX™.
CD22 positive B lymphocytes predominate in the white pulp but are only sparse in the red pulp.

Anti-CD22 antibody [MSVA-022R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04393

GTX04393 IHC-P Image

IHC-P analysis of human ileum mucosa tissue section using GTX04393 CD22 antibody [MSVA-022R] HistoMAX™.
CD22 positive B lymphocytes are rare in this sample of ileum mucosa.

REFERENCE

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REVIEW

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Package List Price ($)
$ 629
$ 299