PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer. Based on the testing result, the pH 7.8 showed a strongest staining pattern. The pH 9.0 is acceptable but lower pH results in a significant deterioration of sensitivity.
|
Not tested in other applications.
Calculated MW
Positive Control
Tonsil: A strong, membranous staining should be seen in the majority of interfollicular lymphocytes but also in a fraction of lymphocytes in the germinal centres and the mantle zone.
Negative Control
Tonsil: CD27 staining should be absent in all epithelial cells and a fraction of lymphocytes.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human CD27 protein fragment (aa 28-170)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD27 molecule , S152 , S152. LPFS2 , T14 , TNFRSF7 , Tp55
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-CD27 antibody [MSVA-027M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04394](/templates/default/js/lightbox/images/loading.gif "Anti-CD27 antibody [MSVA-027M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04394")
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GTX04394 IHC-P Image
IHC-P analysis of human mucosa from duodenum tissue using GTX04394 CD27 antibody [MSVA-027M] HistoMAX™.
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GTX04394 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX04394 CD27 antibody [MSVA-027M] HistoMAX™.
CD27 positive lymphocytes predominate in the interfollicular area.
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GTX04394 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX04394 CD27 antibody [MSVA-027M] HistoMAX™.
Many lymphocytes are CD27 positive but the surface epithelium does not stain.
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REFERENCE
There are currently no references for CD27 antibody [MSVA-027M] HistoMAX™ (GTX04394). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD27 antibody [MSVA-027M] HistoMAX™ (GTX04394). Be the first to share your experience with this product.
![Anti-CD27 antibody [MSVA-027M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04394](/upload/website/prouct_img/small/GTX04394/GTX04394_20230728_IHC-P_19_23072722_482.webp "Anti-CD27 antibody [MSVA-027M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04394")
![Anti-CD27 antibody [MSVA-027M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04394](/upload/website/prouct_img/small/GTX04394/GTX04394_20230728_IHC-P_149_23072722_729.webp "Anti-CD27 antibody [MSVA-027M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04394")
![Anti-CD27 antibody [MSVA-027M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04394](/upload/website/prouct_img/small/GTX04394/GTX04394_20230728_IHC-P_279_23072723_624.webp "Anti-CD27 antibody [MSVA-027M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04394")
![Anti-CD27 antibody [MSVA-027M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04394](/upload/website/prouct_img/normal/GTX04394/GTX04394_20230728_IHC-P_19_23072722_482.webp "Anti-CD27 antibody [MSVA-027M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04394")
![Anti-CD27 antibody [MSVA-027M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04394](/upload/website/prouct_img/normal/GTX04394/GTX04394_20230728_IHC-P_149_23072722_729.webp "Anti-CD27 antibody [MSVA-027M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04394")
![Anti-CD27 antibody [MSVA-027M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04394](/upload/website/prouct_img/normal/GTX04394/GTX04394_20230728_IHC-P_279_23072723_624.webp "Anti-CD27 antibody [MSVA-027M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04394")