PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48 and BOND RX - Leica. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Tonsil: A moderate to strong membranous staining should be seen in all T-cells in the interfollicular T-zones and in the germinal centres (few cells).
Negative Control
Tonsil: All epithelial structures and B-cells should not show any CD3 immunostaining.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for BSA and azide free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide from human CD3e protein (around aa 20-40)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD3e molecule , IMD18 , T3E , TCRE
Cellular Localization
Cell membrane
Background
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-CD3 epsilon antibody [MSVA-003R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04389](/templates/default/js/lightbox/images/loading.gif "Anti-CD3 epsilon antibody [MSVA-003R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04389")
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GTX04389 IHC-P Image
IHC-P analysis of human squamous cell carcinoma tissue section using GTX04389 CD3 epsilon antibody [MSVA-003R] HistoMAX™.
Squamous cell carcinoma containing numerous CD3 epsilon positive T lymphocytes.
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GTX04389 IHC-P Image
IHC-P analysis of human appendix mucosa tissue section using GTX04389 CD3 epsilon antibody [MSVA-003R] HistoMAX™.
In the appendix numerous CD3 epsilon positive T lymphocytes occur in the epithelium the lamina propria and in germinal centres.
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GTX04389 IHC-P Image
IHC-P analysis of human diffuse large B-cell lymphoma tissue section using GTX04389 CD3 epsilon antibody [MSVA-003R] HistoMAX™.
Diffuse large B cell lymphoma infiltrated by numerous CD3 epsilon positive non neoplastic lymphocytes.
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REFERENCE
There are currently no references for CD3 epsilon antibody [MSVA-003R] HistoMAX™ (GTX04389). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD3 epsilon antibody [MSVA-003R] HistoMAX™ (GTX04389). Be the first to share your experience with this product.
![Anti-CD3 epsilon antibody [MSVA-003R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04389](/upload/website/prouct_img/small/GTX04389/GTX04389_20230728_IHC-P_11_23072722_531.webp "Anti-CD3 epsilon antibody [MSVA-003R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04389")
![Anti-CD3 epsilon antibody [MSVA-003R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04389](/upload/website/prouct_img/small/GTX04389/GTX04389_20230728_IHC-P_141_23072722_264.webp "Anti-CD3 epsilon antibody [MSVA-003R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04389")
![Anti-CD3 epsilon antibody [MSVA-003R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04389](/upload/website/prouct_img/small/GTX04389/GTX04389_20230728_IHC-P_271_23072723_288.webp "Anti-CD3 epsilon antibody [MSVA-003R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04389")
![Anti-CD3 epsilon antibody [MSVA-003R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04389](/upload/website/prouct_img/normal/GTX04389/GTX04389_20230728_IHC-P_11_23072722_531.webp "Anti-CD3 epsilon antibody [MSVA-003R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04389")
![Anti-CD3 epsilon antibody [MSVA-003R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04389](/upload/website/prouct_img/normal/GTX04389/GTX04389_20230728_IHC-P_141_23072722_264.webp "Anti-CD3 epsilon antibody [MSVA-003R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04389")
![Anti-CD3 epsilon antibody [MSVA-003R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04389](/upload/website/prouct_img/normal/GTX04389/GTX04389_20230728_IHC-P_271_23072723_288.webp "Anti-CD3 epsilon antibody [MSVA-003R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04389")