PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Liver: At least a weak to moderate staining of the majority of the hepatic sinusoidal endothelial cells should be seen.
Negative Control
Liver: Hepatocytes should not show any CD31 staining.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (around aa 625-738) of human CD31 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
platelet and endothelial cell adhesion molecule 1 , CD31 , CD31/EndoCAM , GPIIA' , PECA1 , PECAM-1 , endoCAM
Cellular Localization
Cell membrane , Cell junction
Background
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]
Database
Research Area
DATA IMAGES
![Anti-CD31 antibody [MSVA-031M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04395](/templates/default/js/lightbox/images/loading.gif "Anti-CD31 antibody [MSVA-031M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04395")
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GTX04395 IHC-P Image
IHC-P analysis of human Kaposi's sarcoma (KS) from skin tissue using GTX04395 CD31 antibody [MSVA-031M] HistoMAX™.
Kaposi sarcoma showing moderate CD31 immunostaining of tumor cells.
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GTX04395 IHC-P Image
IHC-P analysis of human renal cortex tissue using GTX04395 CD31 antibody [MSVA-031M] HistoMAX™.
Strong CD31 staining of capillary endothelium in stroma and glomeruli of the kidney cortex.
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GTX04395 IHC-P Image
IHC-P analysis of human liver tissue using GTX04395 CD31 antibody [MSVA-031M] HistoMAX™.
CD31 is moderately expressed in sinusoids of the liver.
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REFERENCE
There are currently no references for CD31 antibody [MSVA-031M] HistoMAX™ (GTX04395). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD31 antibody [MSVA-031M] HistoMAX™ (GTX04395). Be the first to share your experience with this product.
![Anti-CD31 antibody [MSVA-031M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04395](/upload/website/prouct_img/small/GTX04395/GTX04395_20230728_IHC-P_280_23072723_294.webp "Anti-CD31 antibody [MSVA-031M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04395")
![Anti-CD31 antibody [MSVA-031M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04395](/upload/website/prouct_img/small/GTX04395/GTX04395_20230728_IHC-P_20_23072722_543.webp "Anti-CD31 antibody [MSVA-031M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04395")
![Anti-CD31 antibody [MSVA-031M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04395](/upload/website/prouct_img/small/GTX04395/GTX04395_20230728_IHC-P_150_23072722_344.webp "Anti-CD31 antibody [MSVA-031M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04395")
![Anti-CD31 antibody [MSVA-031M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04395](/upload/website/prouct_img/normal/GTX04395/GTX04395_20230728_IHC-P_280_23072723_294.webp "Anti-CD31 antibody [MSVA-031M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04395")
![Anti-CD31 antibody [MSVA-031M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04395](/upload/website/prouct_img/normal/GTX04395/GTX04395_20230728_IHC-P_20_23072722_543.webp "Anti-CD31 antibody [MSVA-031M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04395")
![Anti-CD31 antibody [MSVA-031M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04395](/upload/website/prouct_img/normal/GTX04395/GTX04395_20230728_IHC-P_150_23072722_344.webp "Anti-CD31 antibody [MSVA-031M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04395")