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CD4 antibody [MSVA-004R] HistoMAX™

Recombinant antibody HistoMAX
Anti-CD4 antibody [MSVA-004R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04379
Anti-CD4 antibody [MSVA-004R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04379
Anti-CD4 antibody [MSVA-004R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04379

Cat. No. GTX04379

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 MSVA-004R

Isotype

 IgG

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($679),
100 μl ($299)
See all CD4 products
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

Tonsil: A moderate to strong membranous CD4 immunostaining should be seen in a subset of T-lymphocytes which is predominantly located in the interfollicular area. Macrophages in the germinal centres should show a moderate CD4 positivity. Liver: Kupffer cells and endothelial cells in the liver sinusoids must show at least a moderate CD4 staining.

Negative Control

Tonsil: B-cells and epithelial cells must not show any CD4 staining. Liver: Hepatocytes must not show any CD4 staining.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa 216-396) of human CD4 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD4 molecule , CD4mut

Cellular Localization

Cell membrane

Background

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES

Anti-CD4 antibody [MSVA-004R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04379

GTX04379 IHC-P Image

IHC-P analysis of human liver tissue using GTX04379 CD4 antibody [MSVA-004R] HistoMAX™.
Endothelial CD4 positivity occurs at moderate intensity in liver sinusoids.

Anti-CD4 antibody [MSVA-004R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04379

GTX04379 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX04379 CD4 antibody [MSVA-004R] HistoMAX™.
In tonsil tissue a strong CD4 immunostaining is seen in a fraction of T cells and in Langerhans cells of the tonsil surface epithelium. Epithelial cells are negative.

Anti-CD4 antibody [MSVA-004R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04379

GTX04379 IHC-P Image

IHC-P analysis of human B-cell chronic lymphocytic leukemia (B-CLL) tissue using GTX04379 CD4 antibody [MSVA-004R] HistoMAX™.
B CLL containing numerous CD4 positive lymphocytes.

REFERENCE

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REVIEW

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Package List Price ($)
$ 679
$ 299