PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
|
Not tested in other applications.
Calculated MW
Positive Control
Tonsil: A strong membranous CD45RA immunostaining should be seen in the majority of lymphocytes.
Negative Control
Tonsil: CD45RA immunostaining should be absent in epithelial cells.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Stimulated human leukocytes
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein tyrosine phosphatase receptor type C , B220 , CD45 , CD45R , GP180 , L-CA , LCA , LY5 , T200
Cellular Localization
Cell membrane
Background
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
Database
Research Area
DATA IMAGES
![Anti-CD45RA antibody [MSVA-472M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04372](/templates/default/js/lightbox/images/loading.gif "Anti-CD45RA antibody [MSVA-472M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04372")
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GTX04372 IHC-P Image
IHC-P analysis of human thymus tissue using GTX04372 CD45RA antibody [MSVA-472M] HistoMAX™.
More than 99 of the cortical thymocytes are CD45RA negative. The rate of CD45RA positive cells is markedly higher in the medulla.
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GTX04372 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX04372 CD45RA antibody [MSVA-472M] HistoMAX™.
The majority of lymphocytes of all types are CD45RA positive in the tonsil while the surface epithelium is CD45RA negative.
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GTX04372 IHC-P Image
IHC-P analysis of human spleen tissue using GTX04372 CD45RA antibody [MSVA-472M] HistoMAX™.
The vast majority of splenic lymphocytes of the white pulp of the spleen are CD45RA positive.
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REFERENCE
There are currently no references for CD45RA antibody [MSVA-472M] HistoMAX™ (GTX04372). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD45RA antibody [MSVA-472M] HistoMAX™ (GTX04372). Be the first to share your experience with this product.
![Anti-CD45RA antibody [MSVA-472M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04372](/upload/website/prouct_img/small/GTX04372/GTX04372_20230728_IHC-P_153_23072722_131.webp "Anti-CD45RA antibody [MSVA-472M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04372")
![Anti-CD45RA antibody [MSVA-472M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04372](/upload/website/prouct_img/small/GTX04372/GTX04372_20230728_IHC-P_283_23072723_970.webp "Anti-CD45RA antibody [MSVA-472M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04372")
![Anti-CD45RA antibody [MSVA-472M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04372](/upload/website/prouct_img/small/GTX04372/GTX04372_20230728_IHC-P_23_23072722_304.webp "Anti-CD45RA antibody [MSVA-472M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04372")
![Anti-CD45RA antibody [MSVA-472M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04372](/upload/website/prouct_img/normal/GTX04372/GTX04372_20230728_IHC-P_153_23072722_131.webp "Anti-CD45RA antibody [MSVA-472M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04372")
![Anti-CD45RA antibody [MSVA-472M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04372](/upload/website/prouct_img/normal/GTX04372/GTX04372_20230728_IHC-P_283_23072723_970.webp "Anti-CD45RA antibody [MSVA-472M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04372")
![Anti-CD45RA antibody [MSVA-472M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04372](/upload/website/prouct_img/normal/GTX04372/GTX04372_20230728_IHC-P_23_23072722_304.webp "Anti-CD45RA antibody [MSVA-472M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04372")