GeneTex

  • Country / Location Selection

United States (US)

CD5 antibody [MSVA-005R] HistoMAX™

Orthogonal HistoMAX
Anti-CD5 antibody [MSVA-005R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04427
Anti-CD5 antibody [MSVA-005R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04427
Anti-CD5 antibody [MSVA-005R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04427

Cat. No. GTX04427

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 MSVA-005R

Isotype

 IgG

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($679),
100 μl ($299)
See all CD5 products
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on the testing result, the pH 9.0 showed a strongest staining pattern. The pH 7.8 is acceptable but lower pH results in a significant reduction of sensitivity.
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

Tonsil: A strong CD5 staining of virtually all T-cells in both T-zones and in germinal centres as well as an at least weak to moderate staining of dispersed B-cells in the mantle zone should be seen.

Negative Control

Tonsil: CD5 staining should be absent in germinal centre B-cells and in surface epithelial cells (A fraction of crypt epithelial cells may stain positive).

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA (Please contact us for BSA and azide free format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa32-372) corresponding to human CD5 protein (extracellular).

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD5 molecule , LEU1 , T1

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

This gene encodes a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2016]

Database

Research Area

DATA IMAGES

Anti-CD5 antibody [MSVA-005R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04427

GTX04427 IHC-P Image

IHC-P analysis of human B-cell chronic lymphocytic leukemia tissue section using GTX04427 CD5 antibody [MSVA-005R] HistoMAX™.
Lymph node B-CLL with strong CD5 positivity of all tumor cells.

Anti-CD5 antibody [MSVA-005R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04427

GTX04427 IHC-P Image

IHC-P analysis of human tonsil tissue section using GTX04427 CD5 antibody [MSVA-005R] HistoMAX™.
Strong CD5 positivity of T lymphocytes interfollicular and in germinal centres.

Anti-CD5 antibody [MSVA-005R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04427

GTX04427 IHC-P Image

IHC-P analysis of human ileum mucosa tissue section using GTX04427 CD5 antibody [MSVA-005R] HistoMAX™.
Strong CD5 immunostaining of T lymphocytes in the ileum mucosa.

REFERENCE

There are currently no references for CD5 antibody [MSVA-005R] HistoMAX™ (GTX04427). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for CD5 antibody [MSVA-005R] HistoMAX™ (GTX04427). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 679
$ 299