PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:100-1:200 |
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
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Not tested in other applications.
Calculated MW
Positive Control
Colon: A strong caldesmon staining should be seen of the muscularis mucosa and of a membrane-like layer along the basement membranes of crypts (intestinal subepithelial myofibroblasts).
Negative Control
Colon: Normal epithelial cells must not show caldesmon staining.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for BSA and azide free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human full-length CALD1 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
caldesmon 1 , CDM , H-CAD , HCAD , L-CAD , LCAD , NAG22
Cellular Localization
Cytoplasm, cytoskeleton
Background
This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX04451 IHC-P Image
IHC-P analysis of human appendix mucosa tissue section using GTX04451 Caldesmon antibody [MSVA-538R] HistoMAX™. Intense caldesmon h positivity of intestinal subepithelial myofibroblasts ISEMFs and of the muscularis mucosae Caldesmon h immunohistochemistry.
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GTX04451 IHC-P Image
IHC-P analysis of human Breast tissue section using GTX04451 Caldesmon antibody [MSVA-538R] HistoMAX™. Caldesmon h staining of myoepithelial cells Caldesmon h immunohistochemistry.
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GTX04451 IHC-P Image
IHC-P analysis of human uterine leiomyosarcoma tissue section using GTX04451 Caldesmon antibody [MSVA-538R] HistoMAX™. Leiomyosarcoma showing intense Caldesmon immunostaining of tumor cells Caldesmon immunohistochemistry.
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REFERENCE
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REVIEW
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