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Caspase 8 antibody - VetSignal

Anti-Caspase 8 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134950
Anti-Caspase 8 antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134950
Anti-Caspase 8 antibody - VetSignal used in Western Blot (WB). GTX134950
Anti-Caspase 8 antibody - VetSignal used in Western Blot (WB). GTX134950

Cat No. GTX134950

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Cat, Dog
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

MDCK

Predict Reactivity

Human(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to dog Caspase 8.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

caspase 8

Database

Research Area

DATA IMAGES
Anti-Caspase 8 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134950

GTX134950 ICC/IF Image

Caspase 8 antibody detects Caspase 8 protein at cytoplasm by immunofluorescent analysis.
Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Caspase 8 stained by Caspase 8 antibody (GTX134950) diluted at 1:100.
Blue: Hoechst 33342 staining.

Anti-Caspase 8 antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134950

GTX134950 IHC-P Image

Caspase 8 antibody detects Caspase 8 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat kidney.
Caspase 8 stained by Caspase 8 antibody (GTX134950) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Caspase 8 antibody - VetSignal used in Western Blot (WB). GTX134950

GTX134950 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody (GTX134950) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Caspase 8 antibody - VetSignal used in Western Blot (WB). GTX134950

GTX134950 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody (GTX134950) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319