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Cops5 antibody

Anti-Cops5 antibody used in Western Blot (WB). GTX127395
Anti-Cops5 antibody used in IHC (Whole mount) (IHC-Wm). GTX127395

Cat No. GTX127395

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-Wm

Reactivity

Zebrafish
Package
200 μl ($409),
50 μl ($219)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

Zebrafish eye , zebrafish embryo

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Cops5

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

COP9 signalosome subunit 5 , jab1 , zgc:73130

Cellular Localization

Cytoplasm , Nucleus

Background

Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of E3 ligase complexes, leading to modify the Ubl ligase activity. In the complex, it probably acts as the catalytic center that mediates the cleavage of nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex.

Database

Research Area

DATA IMAGES

Anti-Cops5 antibody used in Western Blot (WB). GTX127395

GTX127395 WB Image

Sample (30 ug of whole cell lysate)
A: Zebrafish eye
10% SDS PAGE
GTX127395 diluted at 1:1000

Anti-Cops5 antibody used in IHC (Whole mount) (IHC-Wm). GTX127395

GTX127395 IHC-Wm Image

Cops5 antibody detects Cops5 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Cops5 antibody (GTX127395) dilution: 1:100.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 409
$ 219