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Creatine kinase (muscle) antibody - VetSignal

Anti-Creatine kinase (muscle) antibody - VetSignal used in Western Blot (WB). GTX134955
Anti-Creatine kinase (muscle) antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134955
Anti-Creatine kinase (muscle) antibody - VetSignal used in Western Blot (WB). GTX134955

Cat No. GTX134955

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Cat, Dog
Package
100 μl ($329)
See all Creatine kinase (muscle) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

MDCK

Predict Reactivity

Human, Mouse, Rat, Rabbit, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to dog Creatine kinase (muscle).

Purification

Purified by antigen-affinity chromatography.
BSA|Glycerol|PBS|Thimerosal

Conjugation

Unconjugated

RRID

AB_2887399

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

creatine kinase, M-type , CPK-M , M-CK

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-Creatine kinase (muscle) antibody - VetSignal used in Western Blot (WB). GTX134955

GTX134955 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Creatine kinase (muscle) antibody (GTX134955) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Creatine kinase (muscle) antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134955

GTX134955 IHC-P Image

Creatine kinase (muscle) antibody detects Creatine kinase (muscle) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat muscle.
Creatine kinase (muscle) stained by Creatine kinase (muscle) antibody (GTX134955) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Creatine kinase (muscle) antibody - VetSignal used in Western Blot (WB). GTX134955

GTX134955 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Creatine kinase (muscle) antibody (GTX134955) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329