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Cyclin D1 antibody - VetSignal

Anti-Cyclin D1 antibody - VetSignal used in Western Blot (WB). GTX134959
Anti-Cyclin D1 antibody - VetSignal used in Western Blot (WB). GTX134959

Cat No. GTX134959

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Cat, Dog
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

MDCK

Predict Reactivity

Human, Mouse, Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml

Immunogen

The immunogen used to generate this antibody corresponds to dog Cyclin D1.

Purification

Purified by antigen-affinity chromatography.
Glycerol|PBS|ProClin

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cyclin D1 , CCND1 , BCL1 , D11S287E , PRAD1 , U21B31

Background

Essential for the control of the cell cycle at the G1/S (start) transition.
DATA IMAGES
Anti-Cyclin D1 antibody - VetSignal used in Western Blot (WB). GTX134959

GTX134959 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody (GTX134959) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Cyclin D1 antibody - VetSignal used in Western Blot (WB). GTX134959

GTX134959 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody (GTX134959) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319