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Cyclin D1 antibody - VetSignal

Anti-Cyclin D1 antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134959
Anti-Cyclin D1 antibody - VetSignal used in Western Blot (WB). GTX134959
Anti-Cyclin D1 antibody - VetSignal used in Western Blot (WB). GTX134959

Cat No. GTX134959

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Cat, Dog
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

MDCK

Predict Reactivity

Human, Mouse, Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to dog Cyclin D1.

Purification

Purified by antigen-affinity chromatography.
Glycerol|PBS|ProClin

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cyclin D1

Cellular Localization

Nucleus,Cytoplasm,Membrane

Database

Research Area

DATA IMAGES
Anti-Cyclin D1 antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134959

GTX134959 IHC-P Image

Cyclin D1 antibody detects Cyclin D1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat liver.
Cyclin D1 stained by Cyclin D1 antibody (GTX134959) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cyclin D1 antibody - VetSignal used in Western Blot (WB). GTX134959

GTX134959 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody (GTX134959) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Cyclin D1 antibody - VetSignal used in Western Blot (WB). GTX134959

GTX134959 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody (GTX134959) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319