PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
|
Not tested in other applications.
Calculated MW
Positive Control
Skin: A strong nuclear and cytoplasmic Cystatin A immunostaining should be seen in the granular layer.
Negative Control
Colon: No epithelial cell staining should be seen. Few inflammatory cells may show Cystatin A positivity.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human CSTA protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cystatin A , AREI , PSS4 , STF1 , STFA
Cellular Localization
Cytoplasm
Background
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Cystatin A antibody [MSVA-461M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04453](/templates/default/js/lightbox/images/loading.gif "Anti-Cystatin A antibody [MSVA-461M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04453")
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GTX04453 IHC-P Image
IHC-P analysis of human skin tissue using GTX04453 Cystatin A antibody [MSVA-461M] HistoMAX™.
In the skin a nuclear and cytoplasmic Cystatin A immunostaining is predominantly seen in the granular cell layer. The staining intensity decreases towards the basal cell layer.
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GTX04453 IHC-P Image
IHC-P analysis of human esophageal squamous cell carcinoma (ESCC) tissue using GTX04453 Cystatin A antibody [MSVA-461M] HistoMAX™.
Squamous cell carcinoma of the esophagus showing a Cystatin A immunostaining of all tumor cells.
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GTX04453 IHC-P Image
IHC-P analysis of human mucosa from rectum tissue using GTX04453 Cystatin A antibody [MSVA-461M] HistoMAX™.
Cystatin A immunostaining is always absent in epithelial cells of the colon.
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REFERENCE
There are currently no references for Cystatin A antibody [MSVA-461M] HistoMAX™ (GTX04453). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cystatin A antibody [MSVA-461M] HistoMAX™ (GTX04453). Be the first to share your experience with this product.
![Anti-Cystatin A antibody [MSVA-461M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04453](/upload/website/prouct_img/small/GTX04453/GTX04453_20230728_IHC-P_31_23072722_546.webp "Anti-Cystatin A antibody [MSVA-461M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04453")
![Anti-Cystatin A antibody [MSVA-461M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04453](/upload/website/prouct_img/small/GTX04453/GTX04453_20230728_IHC-P_161_23072722_246.webp "Anti-Cystatin A antibody [MSVA-461M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04453")
![Anti-Cystatin A antibody [MSVA-461M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04453](/upload/website/prouct_img/small/GTX04453/GTX04453_20230728_IHC-P_291_23072723_221.webp "Anti-Cystatin A antibody [MSVA-461M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04453")
![Anti-Cystatin A antibody [MSVA-461M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04453](/upload/website/prouct_img/normal/GTX04453/GTX04453_20230728_IHC-P_31_23072722_546.webp "Anti-Cystatin A antibody [MSVA-461M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04453")
![Anti-Cystatin A antibody [MSVA-461M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04453](/upload/website/prouct_img/normal/GTX04453/GTX04453_20230728_IHC-P_161_23072722_246.webp "Anti-Cystatin A antibody [MSVA-461M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04453")
![Anti-Cystatin A antibody [MSVA-461M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04453](/upload/website/prouct_img/normal/GTX04453/GTX04453_20230728_IHC-P_291_23072723_221.webp "Anti-Cystatin A antibody [MSVA-461M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04453")