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Cytokeratin 13 antibody [MSVA-613M] HistoMAX™

HistoMAX
Anti-Cytokeratin 13 antibody [MSVA-613M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04385
Anti-Cytokeratin 13 antibody [MSVA-613M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04385
Anti-Cytokeratin 13 antibody [MSVA-613M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04385

Cat. No. GTX04385

Host

Mouse

Clonality

Monoclonal

Clone Name

MSVA-613M

Isotype

IgG1

Application

IHC-P

Reactivity

Human
Package
500 μl ($519),
100 μl ($299)
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer. Based on testing result, pH 9.0 showed a strongest staining pattern. However, pH 7.8 results in only a slight reduction of the staining intensity as compared to pH 9.0. Therefore, the pH 7.8 was an optimal for manual staining because of the better tissue preservation at pH 7.8 than at pH 9.0.

This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. Please refer to the protocol.

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

Tonsil: All squamous epithelial cells of the surface squamous epithelium (except the basal layer) and a fraction of squamous cells of the crypts should show strong KRT13 staining.

Negative Control

Tonsil: All lymphocytes and blood vessels must not show any KRT13 staining.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

esophageal keratins of rabbit origin

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 13 , CK13 , K13 , WSN2

Background

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 13 antibody [MSVA-613M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04385

GTX04385 IHC-P Image

IHC-P analysis of human oral cavity squamous cell carcinoma (OCSCC) tissue using GTX04385 Cytokeratin 13 antibody [MSVA-613M] HistoMAX™.
Strong cytokeratin 13 immunostaining in a squamous cell carcinoma of the oral cavity.

Anti-Cytokeratin 13 antibody [MSVA-613M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04385

GTX04385 IHC-P Image

IHC-P analysis of human lip tissue using GTX04385 Cytokeratin 13 antibody [MSVA-613M] HistoMAX™.
Strong cytokeratin 13 staining in all suprabasal cell layers of the non keratinizing squamous epithelium of the lip. The basal cell layer is strictly cytokeratin 13 negative.

Anti-Cytokeratin 13 antibody [MSVA-613M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04385

GTX04385 IHC-P Image

IHC-P analysis of human skin tissue using GTX04385 Cytokeratin 13 antibody [MSVA-613M] HistoMAX™.
Absence of cytokeratin 13 staining in the keratinizing squamous epithelium of the skin.

REFERENCE

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REVIEW

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Package List Price ($)
$ 519
$ 299