Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on testing result, pH 9.0 showed a strongest staining pattern. However, pH 9.0 results in only a slight reduction of the staining intensity as compared to pH 9.0. Therefore, the pH 7.8 was an optimal for manual staining because of the better tissue preservation at pH 7.8 than at pH 9.0.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Tonsil: All squamous epithelial cells of the surface squamous epithelium (except the basal layer) and a fraction of squamous cells of the crypts should show strong KRT13 staining.
Negative Control
Tonsil: All lymphocytes and blood vessels must not show any KRT13 staining.
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for BSA and azide free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
esophageal keratins of rabbit origin
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
keratin 13 , CK13 , K13 , WSN2
Background
The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. [provided by RefSeq, Jul 2008]
Database
Research Area
![Anti-Cytokeratin 13 antibody [MSVA-613M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04385](/upload/website/prouct_img/small/GTX04385/GTX04385_20230728_IHC-P_36_23072722_295.webp "Anti-Cytokeratin 13 antibody [MSVA-613M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04385")
![Anti-Cytokeratin 13 antibody [MSVA-613M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04385](/upload/website/prouct_img/small/GTX04385/GTX04385_20230728_IHC-P_166_23072722_326.webp "Anti-Cytokeratin 13 antibody [MSVA-613M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04385")
![Anti-Cytokeratin 13 antibody [MSVA-613M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04385](/upload/website/prouct_img/small/GTX04385/GTX04385_20230728_IHC-P_296_23072723_185.webp "Anti-Cytokeratin 13 antibody [MSVA-613M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04385")
![Anti-Cytokeratin 13 antibody [MSVA-613M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04385](/upload/website/prouct_img/normal/GTX04385/GTX04385_20230728_IHC-P_36_23072722_295.webp "Anti-Cytokeratin 13 antibody [MSVA-613M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04385")
![Anti-Cytokeratin 13 antibody [MSVA-613M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04385](/upload/website/prouct_img/normal/GTX04385/GTX04385_20230728_IHC-P_166_23072722_326.webp "Anti-Cytokeratin 13 antibody [MSVA-613M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04385")
![Anti-Cytokeratin 13 antibody [MSVA-613M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04385](/upload/website/prouct_img/normal/GTX04385/GTX04385_20230728_IHC-P_296_23072723_185.webp "Anti-Cytokeratin 13 antibody [MSVA-613M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04385")