GeneTex
  • Country / Location Selection

United States (US)

Cytokeratin 14 antibody [MSVA-614R] HistoMAX™

Recombinant antibody HistoMAX
Anti-Cytokeratin 14 antibody [MSVA-614R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04377
Anti-Cytokeratin 14 antibody [MSVA-614R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04377
Anti-Cytokeratin 14 antibody [MSVA-614R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04377

Cat. No. GTX04377

Host

Rabbit

Clonality

Monoclonal

Clone Name

MSVA-614R

Isotype

IgG

Application

IHC-P

Reactivity

Human
Package
500 μl ($519),
100 μl ($299)
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer. Based on testing result, pH 9.0 showed a strongest staining pattern. However, pH 7.8 results in only a slight reduction of the staining intensity as compared to pH 9.0. Therefore, the pH 7.8 was an optimal for manual staining because of the better tissue preservation at pH 7.8 than at pH 9.0.

This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. Please refer to the protocol.

Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

Tonsil: A strong KRT14 immunostaining should be seen in the surface and crypt epithelium.

Negative Control

Tonsil: KRT14 immunostaining should be absent in all non-epithelial cells.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human KRT14 fragment (around aa350-472)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 14 , CK14 , EBS3 , EBS4 , K14 , NFJ

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 14 antibody [MSVA-614R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04377

GTX04377 IHC-P Image

IHC-P analysis of human prostate tissue using GTX04377 Cytokeratin 14 antibody [MSVA-614R] HistoMAX™.
Strong cytokeratin 14 positivity in basal cells of the prostate.

Anti-Cytokeratin 14 antibody [MSVA-614R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04377

GTX04377 IHC-P Image

IHC-P analysis of human esophageal squamous cell carcinoma (ESCC) tissue using GTX04377 Cytokeratin 14 antibody [MSVA-614R] HistoMAX™.
Squamous cell carcinoma of the esophagus with strong predominantly basal cytokeratin 14 immunostaining.

Anti-Cytokeratin 14 antibody [MSVA-614R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04377

GTX04377 IHC-P Image

IHC-P analysis of human skin tissue using GTX04377 Cytokeratin 14 antibody [MSVA-614R] HistoMAX™.
Squamous epithelium of skin with strong cytokeratin 14 immunostaining.

REFERENCE

There are currently no references for Cytokeratin 14 antibody [MSVA-614R] HistoMAX™ (GTX04377). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Cytokeratin 14 antibody [MSVA-614R] HistoMAX™ (GTX04377). Be the first to share your experience with this product.
Package List Price ($)
$ 519
$ 299