PRODUCT
Summary
This antibody was validated on 76 different Normal Tissues by IHC-P.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on testing result, pH 9.0 showed a strongest staining pattern. However, pH 9.0 results in only a slight reduction of the staining intensity as compared to pH 9.0. Therefore, the pH 7.8 was an optimal for manual staining because of the better tissue preservation at pH 7.8 than at pH 9.0.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
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Not tested in other applications.
Calculated MW
Positive Control
Tonsil: A strong KRT14 immunostaining should be seen in the surface and crypt epithelium.
Negative Control
Tonsil: KRT14 immunostaining should be absent in all non-epithelial cells.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for BSA and azide free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human KRT14 fragment (around aa350-472)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
keratin 14 , CK14 , EBS3 , EBS4 , K14 , NFJ
Cellular Localization
Cytoplasm , Nucleus
Background
This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Cytokeratin 14 antibody [MSVA-614R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04377](/templates/default/js/lightbox/images/loading.gif "Anti-Cytokeratin 14 antibody [MSVA-614R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04377")
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GTX04377 IHC-P Image
IHC-P analysis of human prostate tissue section using GTX04377 Cytokeratin 14 antibody [MSVA-614R] HistoMAX.
Strong KRT14 positivity in basal cells of the prostate.
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GTX04377 IHC-P Image
IHC-P analysis of human esophageal squamous cell carcinoma tissue section using GTX04377 Cytokeratin 14 antibody [MSVA-614R] HistoMAX.
Squamous cell carcinoma of the esophagus with strong predominantly basal KRT14 immunostaining.
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GTX04377 IHC-P Image
IHC-P analysis of human skin squamous epithelium tissue section using GTX04377 Cytokeratin 14 antibody [MSVA-614R] HistoMAX.
Squamous epithelium of skin with strong KRT14 immunostaining.
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REFERENCE
There are currently no references for Cytokeratin 14 antibody [MSVA-614R] HistoMAX (GTX04377). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cytokeratin 14 antibody [MSVA-614R] HistoMAX (GTX04377). Be the first to share your experience with this product.
![Anti-Cytokeratin 14 antibody [MSVA-614R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04377](/upload/website/prouct_img/small/GTX04377/GTX04377_20230728_IHC-P_37_23072722_879.webp "Anti-Cytokeratin 14 antibody [MSVA-614R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04377")
![Anti-Cytokeratin 14 antibody [MSVA-614R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04377](/upload/website/prouct_img/small/GTX04377/GTX04377_20230728_IHC-P_167_23072722_551.webp "Anti-Cytokeratin 14 antibody [MSVA-614R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04377")
![Anti-Cytokeratin 14 antibody [MSVA-614R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04377](/upload/website/prouct_img/small/GTX04377/GTX04377_20230728_IHC-P_297_23072723_231.webp "Anti-Cytokeratin 14 antibody [MSVA-614R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04377")
![Anti-Cytokeratin 14 antibody [MSVA-614R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04377](/upload/website/prouct_img/normal/GTX04377/GTX04377_20230728_IHC-P_37_23072722_879.webp "Anti-Cytokeratin 14 antibody [MSVA-614R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04377")
![Anti-Cytokeratin 14 antibody [MSVA-614R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04377](/upload/website/prouct_img/normal/GTX04377/GTX04377_20230728_IHC-P_167_23072722_551.webp "Anti-Cytokeratin 14 antibody [MSVA-614R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04377")
![Anti-Cytokeratin 14 antibody [MSVA-614R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04377](/upload/website/prouct_img/normal/GTX04377/GTX04377_20230728_IHC-P_297_23072723_231.webp "Anti-Cytokeratin 14 antibody [MSVA-614R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04377")