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Cytokeratin 5 antibody [MSVA-605M] HistoMAX™

Recombinant antibody HistoMAX
Anti-Cytokeratin 5 antibody [MSVA-605M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04402
Anti-Cytokeratin 5 antibody [MSVA-605M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04402
Anti-Cytokeratin 5 antibody [MSVA-605M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04402

Cat. No. GTX04402

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MSVA-605M

Isotype

 IgG2a

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($519),
100 μl ($299)
See all Cytokeratin 5 products
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on the testing result, the pH 9.0 is optimal but pH 7.8 results in only slightly weaker stainings. At pH 6, the immunostaining is markedly reduced.

This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

Tonsil: A moderate to strong cytoplasmic KRT5 staining should be seen in virtually all squamous epithelial cells. Prostate: A strong cytoplasmic staining should be present in the majority of basal cells.

Negative Control

Tonsil: Lymphatic cells must not show any KRT5 positivity.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa 316-590) of human Cytokeratin 5 (KRT5) protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 5 , CK5 , DDD , DDD1 , EBS2 , K5 , KRT5A

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 5 antibody [MSVA-605M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04402

GTX04402 IHC-P Image

IHC-P analysis of human prostate tissue using GTX04402 Cytokeratin 5 antibody [MSVA-605M] HistoMAX™.
Basal cells of the prostatic glands show strong cytokeratin 5 immunostaining.

Anti-Cytokeratin 5 antibody [MSVA-605M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04402

GTX04402 IHC-P Image

IHC-P analysis of human squamous cell carcinoma (SCC) from lung tissue using GTX04402 Cytokeratin 5 antibody [MSVA-605M] HistoMAX™.
Strong cytokeratin 5 immunostaining in a squamous cell carcinoma of the lung..

Anti-Cytokeratin 5 antibody [MSVA-605M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04402

GTX04402 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX04402 Cytokeratin 5 antibody [MSVA-605M] HistoMAX™.
Squamous epithelium of the tonsil surface shows strong cytokeratin 5 immunostaining of all cell layers.

REFERENCE

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REVIEW

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Package List Price ($)
$ 519
$ 299