GeneTex
United States (US)

DCK antibody

GTX107636_40632_20160721_WB_shRNA_watermark_19011507_325.jpg
GTX107636_40632_20160317_WB_19011506_807.jpg
GTX107636_40632_WB_R_19011506_343.jpg
GTX107636_40632_WB_M_brain_19011506_367.jpg
GTX107636_40632_20150514_IP_19011507_143.jpg
GTX107636_39946_IHC_19011507_410.jpg
GTX107636_IFA_19011507_530.jpg
GTX107636_40632_20160721_WB_19011507_576.jpg

Cat No. GTX107636

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A549 , mouse brain , PC-12 , Rat2

Predict Reactivity

Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DCK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Deoxycytidine Kinase,Dck

Cellular Localization

Nucleus

Background

Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq]

Database

DATA IMAGES
GTX107636_40632_20160721_WB_shRNA_watermark_19011507_325.jpg

GTX107636 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (GTX107636) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107636_40632_20160317_WB_19011506_807.jpg

GTX107636 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (GTX107636) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107636_40632_WB_R_19011506_343.jpg

GTX107636 WB Image

DCK antibody detects DCK protein by western blot analysis.
A. 30 µg PC-12 whole cell extract
B. 30 µg Rat2 whole cell extract
12% SDS-PAGE
DCK antibody (GTX107636) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107636_40632_WB_M_brain_19011506_367.jpg

GTX107636 WB Image

DCK antibody detects DCK protein by western blot analysis.
A. 50 µg mouse brain extract
12% SDS-PAGE
DCK antibody (GTX107636) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107636_40632_20150514_IP_19011507_143.jpg

GTX107636 IP Image

Immunoprecipitation of DCK protein from 293T whole cell extracts using 5 μg of DCK antibody (GTX107636).
Western blot analysis was performed using DCK antibody (GTX107636).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX107636_39946_IHC_19011507_410.jpg

GTX107636 IHC-P Image

DCK antibody detects DCK protein at cytosol and nucleus on human lung carcinoma by immunohistochemical analysis.
Sample: DCK antibody (GTX107636) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107636_IFA_19011507_530.jpg

GTX107636 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Deoxycytidine kinase(GTX107636) antibody at 1:200 dilution.

GTX107636_40632_20160721_WB_19011507_576.jpg

GTX107636 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (GTX107636) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169