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Desmin antibody - VetSignal

Anti-Desmin antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134990
Anti-Desmin antibody - VetSignal used in Western Blot (WB). GTX134990
Anti-Desmin antibody - VetSignal used in Western Blot (WB). GTX134990

Cat No. GTX134990

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Cat, Dog
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

MDCK

Predict Reactivity

Human, Mouse, Rat, Rabbit, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein corresponding to dog Desmin.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Desmin,Des

Cellular Localization

Cytoplasm

Background

Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.

Research Area

DATA IMAGES
Anti-Desmin antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134990

GTX134990 ICC/IF Image

Desmin antibody detects Desmin protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Desmin stained by Desmin antibody (GTX134990) diluted at 1:500.

Anti-Desmin antibody - VetSignal used in Western Blot (WB). GTX134990

GTX134990 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Desmin antibody (GTX134990) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Desmin antibody - VetSignal used in Western Blot (WB). GTX134990

GTX134990 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Desmin antibody (GTX134990) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319