PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:75-1:150 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
|
Not tested in other applications.
Calculated MW
Positive Control
Tonsil: A moderate to strong desmoglein 1 immunostaining should be seen in a fraction of squamous epithelial cells.
Negative Control
Desmoglein 1 immunostaining should be absent in all non-squamous epithelial cell types.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for BSA and azide free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Bovine desmoglein (desmosomal glyco protein I) from nasal epidermis.
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
desmoglein 1 , CDHF4 , DG1 , DSG , EPKHE , EPKHIA , PPKS1 , SPPK1
Cellular Localization
Cell membrane , Cell junction, desmosome
Background
This gene encodes a member of the desmoglein protein subfamily. Desmogleins, along with desmocollins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmosomes are cell-cell junctions that help resist shearing forces and are found in high concentrations in cells subject to mechanical stress. This gene is found in a cluster with other desmoglein family members on chromosome 18. The encoded protein has been identified as a target of auto-antibodies in the autoimmune skin blistering disease pemphigus foliaceus. Disruption of this gene has also been associated with the skin diseases palmoplantar keratoderma and erythroderma. [provided by RefSeq, Feb 2015]
Database
Research Area
DATA IMAGES
![Anti-Desmoglein 1 antibody [MSVA-544M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04464](/templates/default/js/lightbox/images/loading.gif "Anti-Desmoglein 1 antibody [MSVA-544M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04464")
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GTX04464 IHC-P Image
IHC-P analysis of human skin tissue section using GTX04464 Desmoglein 1 antibody [MSVA-544M] HistoMAX™.
Squamous epithelium of the skin showing a strong membranous desmoglein 1 immunostaining.
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GTX04464 IHC-P Image
IHC-P analysis of human squamous cell carcinoma tissue section using GTX04464 Desmoglein 1 antibody [MSVA-544M] HistoMAX™.
Squamous cell carcinoma with variable Dsg1 staining of tumor cells showing only weak staining at the periphery of tumor cell nests.
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GTX04464 IHC-P Image
IHC-P analysis of human oral squamous cell carcinoma tissue section using GTX04464 Desmoglein 1 antibody [MSVA-544M] HistoMAX™.
Squamous cell carcinoma of the oral cavity with strong Dsg1 positivity of most tumor cells.
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REFERENCE
There are currently no references for Desmoglein 1 antibody [MSVA-544M] HistoMAX™ (GTX04464). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Desmoglein 1 antibody [MSVA-544M] HistoMAX™ (GTX04464). Be the first to share your experience with this product.
![Anti-Desmoglein 1 antibody [MSVA-544M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04464](/upload/website/prouct_img/small/GTX04464/GTX04464_20230728_IHC-P_45_23072722_217.webp "Anti-Desmoglein 1 antibody [MSVA-544M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04464")
![Anti-Desmoglein 1 antibody [MSVA-544M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04464](/upload/website/prouct_img/small/GTX04464/GTX04464_20230728_IHC-P_175_23072723_421.webp "Anti-Desmoglein 1 antibody [MSVA-544M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04464")
![Anti-Desmoglein 1 antibody [MSVA-544M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04464](/upload/website/prouct_img/small/GTX04464/GTX04464_20230728_IHC-P_305_23072723_188.webp "Anti-Desmoglein 1 antibody [MSVA-544M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04464")
![Anti-Desmoglein 1 antibody [MSVA-544M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04464](/upload/website/prouct_img/normal/GTX04464/GTX04464_20230728_IHC-P_45_23072722_217.webp "Anti-Desmoglein 1 antibody [MSVA-544M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04464")
![Anti-Desmoglein 1 antibody [MSVA-544M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04464](/upload/website/prouct_img/normal/GTX04464/GTX04464_20230728_IHC-P_175_23072723_421.webp "Anti-Desmoglein 1 antibody [MSVA-544M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04464")
![Anti-Desmoglein 1 antibody [MSVA-544M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04464](/upload/website/prouct_img/normal/GTX04464/GTX04464_20230728_IHC-P_305_23072723_188.webp "Anti-Desmoglein 1 antibody [MSVA-544M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04464")